Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as His7-tagged fusion | Streptomyces coelicolor |
Crystallization (Comment) | Organism |
---|---|
crystals are grown at 18°C by the sitting-drop vapordiffusion method, 1.45 A, 1.92 A and 1.66 A crystal structures of AsnO as apoprotein, Fe2+ complex, and product complex, respectively, with (2S,3S)-3-hydroxyasparagine and succinate | Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.479 | - |
L-asparagine | pH 7.5, 25°C | Streptomyces coelicolor |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | non-heme Fe2+ enzyme | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + 2-oxoglutarate + O2 | Streptomyces coelicolor | the beta-hydroxylated asparagine produced is incorporated at position 9 of the calcium-dependent antibiotic (CDA), an 11-residue non-ribosomally synthesized acidic lipopeptide lactone | (2S,3S)-3-hydroxyasparagine + succinate + CO2 | - |
? | |
L-asparagine + 2-oxoglutarate + O2 | Streptomyces coelicolor A3(2) | the beta-hydroxylated asparagine produced is incorporated at position 9 of the calcium-dependent antibiotic (CDA), an 11-residue non-ribosomally synthesized acidic lipopeptide lactone | (2S,3S)-3-hydroxyasparagine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | Q9Z4Z5 | - |
- |
Streptomyces coelicolor A3(2) | Q9Z4Z5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + 2-oxoglutarate + O2 | the beta-hydroxylated asparagine produced is incorporated at position 9 of the calcium-dependent antibiotic (CDA), an 11-residue non-ribosomally synthesized acidic lipopeptide lactone | Streptomyces coelicolor | (2S,3S)-3-hydroxyasparagine + succinate + CO2 | - |
? | |
L-asparagine + 2-oxoglutarate + O2 | the enzyme exclusively acts on free L-asparagine. It is not active toward D-asparagine | Streptomyces coelicolor | (2S,3S)-3-hydroxyasparagine + succinate + CO2 | - |
? | |
L-asparagine + 2-oxoglutarate + O2 | the beta-hydroxylated asparagine produced is incorporated at position 9 of the calcium-dependent antibiotic (CDA), an 11-residue non-ribosomally synthesized acidic lipopeptide lactone | Streptomyces coelicolor A3(2) | (2S,3S)-3-hydroxyasparagine + succinate + CO2 | - |
? | |
L-asparagine + 2-oxoglutarate + O2 | the enzyme exclusively acts on free L-asparagine. It is not active toward D-asparagine | Streptomyces coelicolor A3(2) | (2S,3S)-3-hydroxyasparagine + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AsnO | - |
Streptomyces coelicolor |
L-asparagine 3-hydroxylase | - |
Streptomyces coelicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.98 | - |
L-asparagine | pH 7.5, 25°C | Streptomyces coelicolor |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.4 | - |
L-asparagine | pH 7.5, 25°C | Streptomyces coelicolor |