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Literature summary for 1.14.11.33 extracted from

  • Li, P.; Gao, S.; Wang, L.; Yu, F.; Li, J.; Wang, C.; Li, J.; Wong, J.
    ABH2 couples regulation of ribosomal DNA transcription with DNA alkylation repair (2013), Cell Rep., 4, 817-829.
    View publication on PubMed
Search for more literature for 1.14.11.33

Cloned(Commentary)

Cloned (Comment) Organism
expression of GFP-tagged enzyme in HeLa cells, localization in nucleoli due to the genuine nucleolar localization signal sequence. Ectopic expression of wild-type ABH2 leads to a 2.5fold increase of pre-rRNA, whereas ectopic expression of D173A mutant only leads to a marginal increase of pre-rRNA Homo sapiens

Protein Variants

Protein Variants Comment Organism
D173A site-directed mutagenesis, the mutant maintains nucleolar localization, but is not able to enhance transcription from the rDNA luciferase reporter. eÉctopic expression of wild-type ABH2 leads to a 2.5fold increase of pre-rRNA, whereas ectopic expression of D173A mutant only leads to a marginal increase of pre-rRNA Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information actinomycin D treatment resulted in nuclear localization of ABH2. DNAse I treatment results in a loss of ABH2 nucleolar localization. ABH2 nucleolar localization is dependent on its association with both DNA and RNA Homo sapiens
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nucleolus nucleolar localization sequence RKRPRR at aa38-aa43 of ABH2, mutation of RKR to AAA within this sequence impaired the nuclear and nucleolar localization of ABH2 in HeLa cells. ABH2 associates with nucleolar proteins NCL, NPM1, and UBF Homo sapiens 5730
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Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N3-methylcytosine + 2-oxoglutarate + O2 Homo sapiens
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 cytosine + formaldehyde + succinate + CO2
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
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Source Tissue

Source Tissue Comment Organism Textmining
HEK-293T cell
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 Homo sapiens
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HeLa cell
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 Homo sapiens
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U2-OS cell
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 Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N3-methylcytosine + 2-oxoglutarate + O2
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 Homo sapiens cytosine + formaldehyde + succinate + CO2
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 ?

Synonyms

Synonyms Comment Organism
ABH2
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 Homo sapiens

General Information

General Information Comment Organism
evolution ABH2 is a member of the ABH family DNA repair dioxygenases Homo sapiens
malfunction ABH2 knockdown impairs rDNA transcription and leads to increased single-stranded and double-stranded DNA breaks that are more pronounced in the rDNA genes, whereas ABH2 overexpression protects cells from methyl-methanesulfonate-induced DNA damage and inhibition of rDNA transcription Homo sapiens
additional information ABH2 associates with DNA repair proteins Ku70/Ku80, overview Homo sapiens
physiological function critical role of ABH2 in maintaining rDNA gene integrity and transcription, the DNA alkylation repair enzyme ABH2/ALKBH2 is highly enriched in the nucleolus. ABH2 overexpression protects cells from methyl-methanesulfonate-induced DNA damage and inhibition of rDNA transcription. In response to massive alkylation damage, ABH2 rapidly redistributes from the nucleolus to nucleoplasm. ABH2 interacts with DNA repair proteins Ku70 and Ku80 as well as nucleolar proteins nucleolin, nucleophosmin 1, and upstream binding factor. ABH2 associates with and promotes rDNA transcription through its DNA repair activity. The physical association between ABH2 and Ku70/Ku80 may help mobilize ABH2 to DSBs to remove alkylation damage and thus facilitate the DNA repair process, or the physical association between ABH2 and Ku70/Ku80 may allow Ku70/Ku80 to sense the potential sites of DSBs through recognition of alkylation damages by ABH2 Homo sapiens