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Literature summary for 1.14.11.33 extracted from
- Iron-catalysed oxidation intermediates captured in a DNA repair dioxygenase (2010), Nature, 468, 330-333.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| AlkB in complex with double-stranded DNA containing 1,N6-ethenoadenine, 3-methylthymine, and 3-methylcytosine, using 100 mM NaCl, 25 mM MgCl2, 100 mM cacodylate (pH 6.5) and 20-24% (w/v) PEG 8K or 4K | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D135A | the mutant displays about 5-15% of wild type activity towards 1-methyladenine at pH 7.0 | Escherichia coli |
| D135N | the mutant displays about 5-15% of wild type activity towards 1-methyladenine at pH 7.0 | Escherichia coli |
| D135S | the mutant displays about 5-15% of wild type activity towards 1-methyladenine at pH 7.0 | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required for activity | Escherichia coli |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P05050 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Mono-Q column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA-1,N6-ethenoadenine + 2-oxoglutarate + O2 | - |
Escherichia coli | ? | - |
? | |
| DNA-1-methyladenine + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
| DNA-3-methylcytosine + 2-oxoglutarate + O2 | preferred substrate of AlkB | Escherichia coli | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
| DNA-3-methylthymine + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-thymine + formaldehyde + succinate + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AlkB | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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