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Literature summary for 1.14.11.33 extracted from

  • Sedgwick, B.; Robins, P.; Lindahl, T.
    Direct removal of alkylation damage from DNA by AlkB and related DNA dioxygenases (2006), Methods Enzymol., 408, 108-120.
    View publication on PubMed
Search for more literature for 1.14.11.33

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli
expressed in Escherichia coli BL21(DE3) cells and in Sf9 insect cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for activity Homo sapiens
Fe2+ required for activity Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P05050
-
-
Homo sapiens Q6NS38 isoform ABH2
-
Homo sapiens Q96Q83 isoform ABH3
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged AlkB is purified by Ni-NTA agarose column chromatography, untagged Alkb is purified by DEAE-cellulose column chromatography, Sepharose column chromatography, and Mono S column chromatography Escherichia coli
His-tagged enzyme is purified by Ni-NTA agarose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA-1-ethyladenine + 2-oxoglutarate + O2
-
 Homo sapiens DNA-adenine + acetaldehyde + succinate + CO2
-
 ir
DNA-1-ethyladenine + 2-oxoglutarate + O2
-
 Escherichia coli DNA-adenine + acetaldehyde + succinate + CO2
-
 ir
DNA-1-methyladenine + 2-oxoglutarate + O2 good substrate Homo sapiens DNA-adenine + formaldehyde + succinate + CO2
-
 ir
DNA-1-methyladenine + 2-oxoglutarate + O2 good substrate Escherichia coli DNA-adenine + formaldehyde + succinate + CO2
-
 ir
DNA-1-methylguanine + 2-oxoglutarate + O2 weak substrate Homo sapiens DNA-guanine + formaldehyde + succinate + CO2
-
 ir
DNA-1-methylguanine + 2-oxoglutarate + O2 weak substrate Escherichia coli DNA-guanine + formaldehyde + succinate + CO2
-
 ir
DNA-3-methylcytosine + 2-oxoglutarate + O2 good substrate Homo sapiens DNA-cytosine + formaldehyde + succinate + CO2
-
 ir
DNA-3-methylcytosine + 2-oxoglutarate + O2 good substrate Escherichia coli DNA-cytosine + formaldehyde + succinate + CO2
-
 ir
DNA-3-methylthymine + 2-oxoglutarate + O2 weak substrate Homo sapiens DNA-thymine + formaldehyde + succinate + CO2
-
 ir
DNA-3-methylthymine + 2-oxoglutarate + O2 weak substrate Escherichia coli DNA-thymine + formaldehyde + succinate + CO2
-
 ir
methylated poly(deoxyadenine) + 2-oxoglutarate + O2
-
 Homo sapiens poly(deoxyadenine) + formaldehyde + succinate + CO2
-
 ir
methylated poly(deoxyadenine) + 2-oxoglutarate + O2
-
 Escherichia coli poly(deoxyadenine) + formaldehyde + succinate + CO2
-
 ir
methylated poly(deoxycytosine) + 2-oxoglutarate + O2
-
 Homo sapiens poly(deoxycytosine) + formaldehyde + succinate + CO2
-
 ir
methylated poly(deoxycytosine) + 2-oxoglutarate + O2
-
 Escherichia coli poly(deoxycytosine) + formaldehyde + succinate + CO2
-
 ir
additional information AlkB binds to single-stranded DNA, but less efficiently to double-stranded DNA Escherichia coli ?
-
 ?
additional information isoform ABH2 shows some preference for methylated poly(dA) over methylated poly(dC) and also prefers a double-stranded substrate. not ABH2 cannot demethylate 1-methyladenine in RNA Homo sapiens ?
-
 ?
additional information isoform ABH2 shows some preference for methylated poly(dC) over methylated poly(dA) and also prefers a single-stranded substrate. Isoform ABH3 can also demethylate 1-methyladenine in RNA Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
ABH2 isoform Homo sapiens
ABH3 isoform Homo sapiens
AlkB
-
 Homo sapiens
AlkB
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
2-oxoglutarate
-
 Homo sapiens
2-oxoglutarate
-
 Escherichia coli