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Literature summary for 1.14.11.30 extracted from

  • Hangasky, J.A.; Taabazuing, C.Y.; Martin, C.B.; Eron, S.J.; Knapp, M.J.
    The facial triad in the alpha-ketoglutarate dependent oxygenase FIH A role for sterics in linking substrate binding to O2 activation (2017), J. Inorg. Biochem., 166, 26-33 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.30

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M HEPES, 1.2 M (NH4)2SO4, and 3% PEG 400, pH 7.0 Homo sapiens

Protein Variants

Protein Variants Comment Organism
D201A inactive Homo sapiens
D201E the mutant shows greatly impaired activity compared to the wild type enzyme Homo sapiens
D201G the mutant shows reduced activity compared to the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 2-oxoglutarate wild type enzyme, at pH 7.0 and 37°C Homo sapiens
0.023
-
 2-oxoglutarate mutant enzyme D210G, at pH 7.0 and 37°C Homo sapiens
0.026
-
 2-oxoglutarate mutant enzyme D210E, at pH 7.0 and 37°C Homo sapiens
0.07
-
 O2 mutant enzyme D210E, at pH 7.0 and 37°C Homo sapiens
0.07
-
 DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN mutant enzyme D210E, at pH 7.0 and 37°C Homo sapiens
0.071
-
 DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN mutant enzyme D210G, at pH 7.0 and 37°C Homo sapiens
0.078
-
 DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN wild type enzyme, at pH 7.0 and 37°C Homo sapiens
0.115
-
 O2 mutant enzyme D210G, at pH 7.0 and 37°C Homo sapiens
0.2
-
 O2 wild type enzyme, at pH 7.0 and 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ dependent on Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O2 Homo sapiens hydroxylation at Asn803 hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NWT6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN + 2-oxoglutarate + O2
-
 Homo sapiens DESGLPQLTSYDAEV-(3S)-3-hydroxy-L-asparaginyl-APIQGSRNLLQGEELLRALDQVN + succinate + CO2
-
 ?
hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O2 hydroxylation at Asn803 Homo sapiens hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
factor inhibiting hypoxia inducible factor-1alpha
-
 Homo sapiens
FIH
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0135
-
 O2 mutant enzyme D210E, at pH 7.0 and 37°C Homo sapiens
0.027
-
 DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN mutant enzyme D210E, at pH 7.0 and 37°C Homo sapiens
0.33
-
 O2 mutant enzyme D210G, at pH 7.0 and 37°C Homo sapiens
0.383
-
 DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN mutant enzyme D210G, at pH 7.0 and 37°C Homo sapiens
0.55
-
 O2 wild type enzyme, at pH 7.0 and 37°C Homo sapiens
0.55
-
 DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN wild type enzyme, at pH 7.0 and 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.23
-
 O2 mutant enzyme D210E, at pH 7.0 and 37°C Homo sapiens
0.37
-
 DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN mutant enzyme D210E, at pH 7.0 and 37°C Homo sapiens
2.83
-
 O2 wild type enzyme, at pH 7.0 and 37°C Homo sapiens
3
-
 O2 mutant enzyme D210G, at pH 7.0 and 37°C Homo sapiens
5.7
-
 DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN mutant enzyme D210G, at pH 7.0 and 37°C Homo sapiens
7
-
 DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN wild type enzyme, at pH 7.0 and 37°C Homo sapiens