Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | activates, Km: 0.14 mM | Homo sapiens | |
ascorbate | activates, Km: 0.17 mM | Homo sapiens | |
ascorbate | activates, Km: 0.18 mM | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Spodoptera frugiperda Sf9 cells and Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R367K | inactive mutant of HIF-P4H-1 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-carboxy-4-oxo-3,4-dihydro-1,10-phenanthroline | - |
Homo sapiens | |
3-hydroxypyridine-2-carbonyl-glycine | - |
Homo sapiens | |
N-((3-hydroxy-6-chloroquinolin-2-yl)carbonyl)glycine | - |
Homo sapiens | |
oxalylglycine | - |
Homo sapiens | |
Pyridine-2,4-dicarboxylate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
DLDLEMLAPYIPMDDDFQLRSFDQ | pH 7.8, 37°C | Homo sapiens | |
0.006 | - |
DALDLEMLAPYISMDDDFQL | pH 7.8, 37°C | Homo sapiens | |
0.007 | - |
DLEMLAPYIPMDDDFQL | pH 7.8, 37°C | Homo sapiens | |
0.007 | - |
DLDLEMLAPYIPMDDDFQL | pH 7.8, 37°C | Homo sapiens | |
0.007 | - |
EMLAPYIPMDDDFQL | pH 7.8, 37°C | Homo sapiens | |
0.007 | - |
DLDLEMLAPYIPMDDDF | pH 7.8, 37°C | Homo sapiens | |
0.007 | - |
DALDLEMLAPYISMDDDFQL | pH 7.8, 37°C | Homo sapiens | |
0.008 | - |
DLDLEMLAPYIPMDDDFQL | pH 7.8, 37°C | Homo sapiens | |
0.008 | - |
DLDLEMLAPYIPMDDDFQLRSFDQ | pH 7.8, 37°C | Homo sapiens | |
0.009 | - |
DLDLEMLAPYIPMDD | pH 7.8, 37°C | Homo sapiens | |
0.01 | - |
DLDLEMLAPAIPMDDDFQL | pH 7.8, 37°C | Homo sapiens | |
0.011 | - |
ELDLETLAPYIPMDGEDFQ | pH 7.8, 37°C | Homo sapiens | |
0.014 | - |
DLEMLAPYIPMDDDFQL | pH 7.8, 37°C | Homo sapiens | |
0.015 | - |
DALDLEMLAPYISMDDDFQL | pH 7.8, 37°C | Homo sapiens | |
0.015 | - |
LAPYIPMDDDFQL | pH 7.8, 37°C | Homo sapiens | |
0.02 | - |
DLDLEMLAPAIPMDDDFQL | pH 7.8, 37°C | Homo sapiens | |
0.02 | - |
DLDLEMLAPGIPMDDDFQL | pH 7.8, 37°C | Homo sapiens | |
0.03 | - |
DLDLEMLAPYIPMD | pH 7.8, 37°C | Homo sapiens | |
0.03 | - |
ELDLETLAPYIPMDGEDFQ | pH 7.8, 37°C | Homo sapiens | |
0.035 | - |
EMLAPYIPMDDDFQL | pH 7.8, 37°C | Homo sapiens | |
0.05 | - |
DLDLEMLAPYIPMDDDF | pH 7.8, 37°C | Homo sapiens | |
0.05 | - |
EMLAPYIPMDD | pH 7.8, 37°C | Homo sapiens | |
0.055 | - |
2-oxoglutarate | pH 7.8, 37°C | Homo sapiens | |
0.06 | - |
2-oxoglutarate | pH 7.8, 37°C | Homo sapiens | |
0.06 | - |
DLDLEMLAPYIPMDD | pH 7.8, 37°C | Homo sapiens | |
0.06 | - |
EPEELAQLAPTPGDAIISLD | pH 7.8, 37°C | Homo sapiens | |
0.07 | - |
DLDLEMLAPYIPMDDDF | pH 7.8, 37°C | Homo sapiens | |
0.07 | - |
EEPDLSCLAPFVDTYDMMQM | pH 7.8, 37°C | Homo sapiens | |
0.08 | - |
EMLAPYIPMDDDFQL | pH 7.8, 37°C | Homo sapiens | |
0.1 | - |
DLDLEMLAPYIPMDD | pH 7.8, 37°C | Homo sapiens | |
0.1 | - |
DLDLEMLAPYIPMD | pH 7.8, 37°C | Homo sapiens | |
0.1 | - |
EPEELAQLAPTPGDAIISLD | pH 7.8, 37°C | Homo sapiens | |
0.1 | - |
EEPDLSCLAPFVDTYDMMQM | pH 7.8, 37°C | Homo sapiens | |
0.13 | - |
DALTLLAPAAGDTIISLFG | pH 7.8, 37°C | Homo sapiens | |
0.23 | - |
O2 | pH 7.8, 37°C | Homo sapiens | |
0.25 | - |
O2 | pH 7.8, 37°C | Homo sapiens | |
0.38 | - |
DALTLLAPAAGDTIISLFG | pH 7.8, 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxia-inducible factor-L-proline + 2-oxoglutarate + O2 | Homo sapiens | - |
hypoxia-inducible factor-(4R)-4-hydroxy-L-proline + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | Q9GZT9 | HIF-P4H-2 | - |
Homo sapiens | Q9H6Z9 | HIF-P4H-3 | - |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | expression level of of HIF-P4H-3 mRNA is highest in the adult heart, brain, placenta, lung, and skeletal muscle and in the fetal heart, spleen, and skeletal muscle | Homo sapiens | - |
brain | the level of HIF-P4H-1 mRNA expression is highest in the adult brain, placenta, lung, and kidney | Homo sapiens | - |
brain | the levels of HIF-P4H-2 mRNA expression is highest in the adult heart, brain, lung, and liver and in the fetal brain, heart, spleen, and skeletal muscle | Homo sapiens | - |
heart | expression level of of HIF-P4H-3 mRNA is highest in the adult heart, brain, placenta, lung, and skeletal muscle and in the fetal heart, spleen, and skeletal muscle | Homo sapiens | - |
heart | the levels of HIF-P4H-2 mRNA expression is highest in the adult heart, brain, lung, and liver and in the fetal brain, heart, spleen, and skeletal muscle | Homo sapiens | - |
kidney | the level of HIF-P4H-1 mRNA expression is highest in the adult brain, placenta, lung, and kidney | Homo sapiens | - |
liver | the levels of HIF-P4H-2 mRNA expression is highest in the adult heart, brain, lung, and liver and in the fetal brain, heart, spleen, and skeletal muscle | Homo sapiens | - |
lung | expression level of of HIF-P4H-3 mRNA is highest in the adult heart, brain, placenta, lung, and skeletal muscle and in the fetal heart, spleen, and skeletal muscle | Homo sapiens | - |
lung | the level of HIF-P4H-1 mRNA expression is highest in the adult brain, placenta, lung, and kidney | Homo sapiens | - |
lung | the levels of HIF-P4H-2 mRNA expression is highest in the adult heart, brain, lung, and liver and in the fetal brain, heart, spleen, and skeletal muscle | Homo sapiens | - |
placenta | expression level of of HIF-P4H-3 mRNA is highest in the adult heart, brain, placenta, lung, and skeletal muscle and in the fetal heart, spleen, and skeletal muscle | Homo sapiens | - |
placenta | the level of HIF-P4H-1 mRNA expression is highest in the adult brain, placenta, lung, and kidney | Homo sapiens | - |
skeletal muscle | expression level of of HIF-P4H-3 mRNA is highest in the adult heart, brain, placenta, lung, and skeletal muscle and in the fetal heart, spleen, and skeletal muscle | Homo sapiens | - |
skeletal muscle | the levels of HIF-P4H-2 mRNA expression is highest in the adult heart, brain, lung, and liver and in the fetal brain, heart, spleen, and skeletal muscle | Homo sapiens | - |
spleen | expression level of of HIF-P4H-3 mRNA is highest in the adult heart, brain, placenta, lung, and skeletal muscle and in the fetal heart, spleen, and skeletal muscle | Homo sapiens | - |
spleen | the levels of HIF-P4H-2 mRNA expression is highest in the adult heart, brain, lung, and liver and in the fetal brain, heart, spleen, and skeletal muscle | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DALDLEMLAPYISMDDDFQL + 2-oxoglutarate + O2 | a HIF-3alpha peptide. Vmax is 120% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | ? | - |
? | |
DALDLEMLAPYISMDDDFQL + 2-oxoglutarate + O2 | a HIF-3alpha peptide. Vmax is 120% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | DALDLEMLA-((4R)-4-hydroxy-L-proline)-YISMDDDFQL + succinate + CO2 | - |
? | |
DALDLEMLAPYISMDDDFQL + 2-oxoglutarate + O2 | a HIF-3alpha peptide. Vmax is 150% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | DALDLEMLA-((4R)-4-hydroxy-L-proline)-YISMDDDFQL + succinate + CO2 | - |
? | |
DALTLLAPAAGDTIISLFG + 2-oxoglutarate + O2 | N-terminal hydroxylation site of HIF-1alpha, Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | DALTLLA-((4R)-4-hydroxy-L-proline)-AAGDTIISLFG + succinate + CO2 | - |
? | |
DALTLLAPAAGDTIISLFG + 2-oxoglutarate + O2 | N-terminal hydroxylation site of HIF-1alpha, Vmax is 60% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | DALTLLA-((4R)-4-hydroxy-L-proline)-AAGDTIISLFG + succinate + CO2 | - |
? | |
DLDLEMLAPAIPMDDDFQL + 2-oxoglutarate + O2 | Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | DLDLEMLA-((4R)-4-hydroxy-L-proline)-AIPMDDDFQL + succinate + CO2 | - |
? | |
DLDLEMLAPGIPMDDDFQL + 2-oxoglutarate + O2 | Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | DLDLEMLA-((4R)-4-hydroxy-L-proline)-GIPMDDDFQL + succinate + CO2 | - |
? | |
DLDLEMLAPYIPMD + 2-oxoglutarate + O2 | Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | DLDLEMLA-((4R)-4-hydroxy-L-proline)-YIPMD + succinate + CO2 | - |
? | |
DLDLEMLAPYIPMDD + 2-oxoglutarate + O2 | Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | DLDLEMLA-((4R)-4-hydroxy-L-proline)-YIPMDD + succinate + CO2 | - |
? | |
DLDLEMLAPYIPMDDDF + 2-oxoglutarate + O2 | Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | DLDLEMLA-((4R)-4-hydroxy-L-proline)-YIPMDDDF + succinate + CO2 | - |
? | |
DLDLEMLAPYIPMDDDFQL + 2-oxoglutarate + O2 | - |
Homo sapiens | DLDLEMLA-((4R)-4-hydroxy-L-proline)-YIPMDDDFQL + succinate + CO2 | - |
? | |
DLDLEMLAPYIPMDDDFQLRSFDQ + 2-oxoglutarate + O2 | Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | DLDLEMLA-((4R)-4-hydroxy-L-proline)-YIPMDDDFQLRSFDQ + succinate + CO2 | - |
? | |
DLEMLAPYIPMDDDFQL + 2-oxoglutarate + O2 | Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | DLEMLA-((4R)-4-hydroxy-L-proline)-YIPMDDDFQL + succinate + CO2 | - |
? | |
EEPDLSCLAPFVDTYDMMQM + 2-oxoglutarate + O2 | hydroxylation site of Caenorhabditis elegans HIF-alpha. Vmax is 60% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | ? | - |
? | |
EEPDLSCLAPFVDTYDMMQM + 2-oxoglutarate + O2 | hydroxylation site of Caenorhabditis elegans HIF-alpha. Vmax is 80% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | ? | - |
? | |
ELDLETLAPYIPMDGEDFQ + 2-oxoglutarate + O2 | C-terminal hydroxylation site of HIF-2alpha. Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | ? | - |
? | |
ELDLETLAPYIPMDGEDFQ + 2-oxoglutarate + O2 | C-terminal hydroxylation site of HIF-2alpha. Vmax is 70% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | ? | - |
? | |
ELDLETLAPYIPMDGEDFQ + 2-oxoglutarate + O2 | C-terminal hydroxylation site of HIF-2alpha. Vmax is 80% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | ELDLETLA-((4R)-4-hydroxy-L-proline)-YIPMDGEDFQ + succinate + CO2 | - |
? | |
EMLAPYIPMDD + 2-oxoglutarate + O2 | Vmax is 30% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | EMLA-((4R)-4-hydroxy-L-proline)-YIPMDD + succinate + CO2 | - |
? | |
EMLAPYIPMDDDFQL + 2-oxoglutarate + O2 | Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | EMLA-((4R)-4-hydroxy-L-proline)-YIPMDDDFQL + succinate + CO2 | - |
? | |
EMLAPYIPMDDDFQL + 2-oxoglutarate + O2 | Vmax is 80% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | EMLA-((4R)-4-hydroxy-L-proline)-YIPMDDDFQL + succinate + CO2 | - |
? | |
EPEELAQLAPTPGDAIISLD + 2-oxoglutarate + O2 | N-terminal hydroxylation site of HIF-2alpha. Vmax is 30% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | ? | - |
? | |
EPEELAQLAPTPGDAIISLD + 2-oxoglutarate + O2 | N-terminal hydroxylation site of HIF-2alpha. Vmax is 70% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | ? | - |
? | |
EPEELAQLAPTPGDAIISLD + 2-oxoglutarate + O2 | N-terminal hydroxylation site of HIF-2alpha. Vmax is 80% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | ? | - |
? | |
hypoxia-inducible factor-L-proline + 2-oxoglutarate + O2 | - |
Homo sapiens | hypoxia-inducible factor-(4R)-4-hydroxy-L-proline + succinate + CO2 | - |
? | |
LAPYIPMDDDFQL + 2-oxoglutarate + O2 | Vmax is 90% of the activity with DLDLEMLAPYIPMDDDFQL | Homo sapiens | LA-((4R)-4-hydroxy-L-proline)-YIPMDDDFQL + succinate + CO2 | - |
? | |
additional information | the enzyme requires long peptide substrates. No hydroxylation of: Leu-Ala-Pro, Leu-Ala-Pro-Tyr, Leu-Glu-Met-Leu-Ala-Pro, and Leu-Glu-Met-Leu-Ala-Pro-Tyr | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HIF-P4H-1 | - |
Homo sapiens |
HIF-P4H-2 | - |
Homo sapiens |
HIF-P4H-3 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ki-values above 0.3 mM: pyridine-2,5-dicarboxylate and 3,4-dihydroxybenzoic acid | Homo sapiens | |
0.0002 | - |
N-((3-hydroxy-6-chloroquinolin-2-yl)carbonyl)glycine | pH 7.8, 37°C | Homo sapiens | |
0.0008 | - |
N-((3-hydroxy-6-chloroquinolin-2-yl)carbonyl)glycine | pH 7.8, 37°C | Homo sapiens | |
0.001 | - |
3-hydroxypyridine-2-carbonyl-glycine | pH 7.8, 37°C | Homo sapiens | |
0.002 | - |
3-hydroxypyridine-2-carbonyl-glycine | pH 7.8, 37°C | Homo sapiens | |
0.007 | - |
Pyridine-2,4-dicarboxylate | pH 7.8, 37°C | Homo sapiens | |
0.008 | - |
Pyridine-2,4-dicarboxylate | pH 7.8, 37°C | Homo sapiens | |
0.008 | - |
oxalylglycine | pH 7.8, 37°C | Homo sapiens | |
0.01 | - |
oxalylglycine | pH 7.8, 37°C | Homo sapiens | |
0.01 | - |
3-carboxy-4-oxo-3,4-dihydro-1,10-phenanthroline | pH 7.8, 37°C | Homo sapiens | |
0.015 | - |
3-hydroxypyridine-2-carbonyl-glycine | pH 7.8, 37°C | Homo sapiens | |
0.03 | - |
3-carboxy-4-oxo-3,4-dihydro-1,10-phenanthroline | pH 7.8, 37°C | Homo sapiens | |
0.04 | - |
Pyridine-2,4-dicarboxylate | pH 7.8, 37°C | Homo sapiens | |
0.05 | - |
oxalylglycine | pH 7.8, 37°C | Homo sapiens |