Cloned (Comment) | Organism |
---|---|
gene P3H, DNA and amino acid sequence determination and analysis, preparation of a synthetic gene encoding proline 3-hydroxylase and cloning and overexpression of proline 3-hydroxylase protein in Escherichia coli strain BL21(DE3) resulting in strain SC16497, biotransformation rates, overview | Streptomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | activates, required | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33154 | - |
x * 33154, sequence calculation | Streptomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-proline + 2-oxoglutarate + O2 | Streptomyces sp. | - |
cis-3-hydroxy-L-proline + succinate + CO2 | - |
? | |
L-proline + 2-oxoglutarate + O2 | Streptomyces sp. TH1 | - |
cis-3-hydroxy-L-proline + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
- |
- |
Streptomyces sp. TH1 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant biotransformation of L-proline to cis-3-hydroxy-L-proline in Escherichia coli cells transformed with a synthetic P3H gene, overview | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-proline + 2-oxoglutarate + O2 | - |
Streptomyces sp. | cis-3-hydroxy-L-proline + succinate + CO2 | - |
? | |
L-proline + 2-oxoglutarate + O2 | - |
Streptomyces sp. | cis-3-hydroxy-L-proline + succinate + CO2 | product identification and analysis by NMR spectroscopy | ? | |
L-proline + 2-oxoglutarate + O2 | - |
Streptomyces sp. TH1 | cis-3-hydroxy-L-proline + succinate + CO2 | - |
? | |
L-proline + 2-oxoglutarate + O2 | - |
Streptomyces sp. TH1 | cis-3-hydroxy-L-proline + succinate + CO2 | product identification and analysis by NMR spectroscopy | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33154, sequence calculation | Streptomyces sp. |
Synonyms | Comment | Organism |
---|---|---|
L-proline-3-hydroxylase | - |
Streptomyces sp. |
P3H | - |
Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | 30 | assay at | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 7.9 | assay at | Streptomyces sp. |