Literature summary for 1.14.11.26 extracted from

Baldwin, J.E.; Adlington, R.M.; Coates, J.B.; Crabbe, M.J.; Crouch, N.P.; Keeping, J.W.; Knight, G.C.; Schofield, C.J.; Ting, H.H.; Vallejo, C.A.; et al.
Purification and initial characterization of an enzyme with deacetoxycephalosporin C synthetase and hydroxylase activities (1987), Biochem. J., 245, 831-841.

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Activating Compound

Activating Compound	Comment	Organism	Structure
ammonium chloride	-	Acremonium chrysogenum
ammonium sulfate	-	Acremonium chrysogenum
ascorbate	required	Acremonium chrysogenum
dithiothreitol	up to 30% activation in presence of dithiothreitol	Acremonium chrysogenum

General Stability

General Stability	Organism
sodium phosphate and Bis/Tris buffers are inhibitory	Acremonium chrysogenum

Inhibitors

Inhibitors	Comment	Organism	Structure
(2S,4S,6R,7R)-1-aza-7-((5R)-5-carboxypentanamidol)-4-methyl-8-oxo-5-thiatricyclo-(4,2,0,0)octane-2-carboxylate	reversible, 0.04 mM, 90% inhibition	Acremonium chrysogenum
ammonium hydrogencarbonate	100-500 mM, complete inactivation	Acremonium chrysogenum
bathophenanthroline	-	Acremonium chrysogenum
N-ethylmaleimide	-	Acremonium chrysogenum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	x * 40000, SDS-PAGE	Acremonium chrysogenum

Organism

Organism	UniProt	Comment	Textmining
Acremonium chrysogenum	-	enzyme catalyzes both reaction of EC 1.14.20.1 and of EC 1.14.11.26	-

Purification (Commentary)

Purification (Comment)	Organism
-	Acremonium chrysogenum

Storage Stability

Storage Stability	Organism
-70°C, presence of dithiothreitol, stable for 4 weeks	Acremonium chrysogenum
4°C, presence of dithiothreitol, half-life is 48 h	Acremonium chrysogenum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
deacetoxycephalosporin C + 2-oxoglutarate + O2	-	Acremonium chrysogenum	deacetylcephalosporin C + succinate + CO2 + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 40000, SDS-PAGE	Acremonium chrysogenum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Acremonium chrysogenum

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Release 2023.1 (January 2023)