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Literature summary for 1.14.11.20 extracted from
- Isolation and characterization of a 2-oxoglutarate dependent dioxygenase involved in the second-to-last step in vindoline biosynthesis (1990), Plant Physiol., 94, 1323-1329.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbic acid | in vitro required for maximal activity | Catharanthus roseus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
gel filtration | Catharanthus roseus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Catharanthus roseus | - |
enzyme is induced after exposure of seedlings to light | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Catharanthus roseus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| desacetoxyvindoline + 2-oxoglutarate + O2 | strictly specific for position 4, no hydroxylation of indole alkaloid substrates with a 2,3-double bond | Catharanthus roseus | desacetylvindoline + succinate + CO2 | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Catharanthus roseus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | absolutely required for activity | Catharanthus roseus | |
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Release 2023.1 (January 2023)
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