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Literature summary for 1.14.11.2 extracted from

  • Pektas, S.; Knapp, M.J.
    Substrate preference of the HIF-prolyl hydroxylase-2 (PHD2) and substrate-induced conformational change (2013), J. Inorg. Biochem., 126, 55-60.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.2

Cloned(Commentary)

Cloned (Comment) Organism
recombinant human PHD2 (corresponding to residues 178-426 of the full-length PHD2 sequence) is expressed as a GST-fusion protein in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 DLDLEALAPYIPADDDFQL substrate: CODD peptide, pH 7, 37°C, without NaCl Homo sapiens
0.001
-
 DALTLLAPAAGDTIISLDYG substrate: NODD peptide, pH 7, 37°C, without NaCl Homo sapiens
0.0042
-
 DLDLEALAPYIPADDDFQL substrate: CODD peptide, pH 7, 37°C, 100 mM NaCl Homo sapiens
0.0042
-
 DALTLLAPAAGDTIISLDYG substrate: NODD peptide, pH 7, 37°C, 100 mM NaCl Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DALTLLAPAAGDTIISLDYG + 2-oxoglutarate + O2 NODD peptide derived from native HIF-1alpha395-414 Homo sapiens ?
-
 ?
DLDLEALAPYIPADDDFQL + 2-oxoglutarate + O2 CODD peptide derived from native HIF-1alpha556-574. PHD2 preferred CODD by 20fold over NODD Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
PHD2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0101
-
 DALTLLAPAAGDTIISLDYG substrate: NODD peptide, pH 7, 37°C, without NaCl Homo sapiens
0.0103
-
 DALTLLAPAAGDTIISLDYG substrate: NODD peptide, pH 7, 37°C, 100 mM NaCl Homo sapiens
0.0188
-
 DLDLEALAPYIPADDDFQL substrate: CODD peptide, pH 7, 37°C, without NaCl Homo sapiens
0.02
-
 DLDLEALAPYIPADDDFQL substrate: CODD peptide, pH 7, 37°C, 100 mM NaCl Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.45
-
 DALTLLAPAAGDTIISLDYG substrate: NODD peptide, pH 7, 37°C, 100 mM NaCl Homo sapiens
4.76
-
 DLDLEALAPYIPADDDFQL substrate: CODD peptide, pH 7, 37°C, 100 mM NaCl Homo sapiens
10.1
-
 DALTLLAPAAGDTIISLDYG substrate: NODD peptide, pH 7, 37°C, without NaCl Homo sapiens
18.8
-
 DLDLEALAPYIPADDDFQL substrate: CODD peptide, pH 7, 37°C, without NaCl Homo sapiens