Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Dictyostelium discoideum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dihydroxybenzoate | - |
Dictyostelium discoideum | |
3,4-dihydroxyphenyl acetate | IC50: 0.3 mM | Dictyostelium discoideum | |
CoCl2 | IC50: 0.00025 mM | Dictyostelium discoideum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00002 | - |
His10-rSkp1A | - |
Dictyostelium discoideum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Dictyostelium discoideum | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
SDS-PAGE | Dictyostelium discoideum |
33350 | - |
calculated from sequence of cDNA | Dictyostelium discoideum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | Q86KR9 | - |
- |
Dictyostelium discoideum AX3 | Q86KR9 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose fast flow anion exchange chromatography, phenyl-Sepharose fast flow column chromatography and Source 15Q anion exchange chromatography | Dictyostelium discoideum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
His10-rSkp1A + ? | - |
Dictyostelium discoideum | ? | - |
? | |
His10-rSkp1A + ? | - |
Dictyostelium discoideum AX3 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hypoxia-inducible factor -1alpha-type prolyl 4-hydroxylase | - |
Dictyostelium discoideum |
P4H1 | - |
Dictyostelium discoideum |
prolyl-4-hydroxylase | - |
Dictyostelium discoideum |
Skp1 prolyl hydroxylase | - |
Dictyostelium discoideum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | dependent on physiological concentrations of ascorbate | Dictyostelium discoideum | |
O2 | dependent on physiological concentrations of O2 | Dictyostelium discoideum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Dictyostelium discoideum | predicted value according to the amino acid sequence | - |
6.9 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00025 | - |
IC50: 0.00025 mM | Dictyostelium discoideum | CoCl2 | |
0.3 | - |
IC50: 0.3 mM | Dictyostelium discoideum | 3,4-dihydroxyphenyl acetate |