Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.11.2 extracted from

  • ekkala, M.; Hieta, R.; Kursula, P.; Kivirikko, K.I.; Wierenga, R.K.; Myllyharju, J.
    Crystallization of the proline-rich-peptide binding domain of human type I collagen prolyl 4-hydroxylase (2003), Acta Crystallogr. Sect. D, 59, 940-942.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression, of alpha (I) subunit in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour diffusion, 0.002 ml of 10 mg/ml protein solution in 20 mM bis-Tris, 100 mM glycine, pH 6.8 is mixed with 0.002 ml reservoir solution containing 1.2-1.7 M ammonium phosphate, pH 8.2-8.6, crystals diffract to 3.0 A Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for activity Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
procollagen L-proline + 2-oxoglutarate + O2 Homo sapiens
-
procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-P4H alpha(I) subunit Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
procollagen L-proline + 2-oxoglutarate + O2
-
Homo sapiens procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
?

Subunits

Subunits Comment Organism
tetramer alpha2beta2 Homo sapiens

Synonyms

Synonyms Comment Organism
alpha (I) subunit
-
Homo sapiens
type I C-P4H
-
Homo sapiens