Activating Compound | Comment | Organism | Structure |
---|---|---|---|
catalase | activation | Streptomyces griseoviridis | |
pyridine 2,6-dicarboxylate | activator | Streptomyces griseoviridis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | at concentrations higher than 0.5 mM, decreases activity | Streptomyces griseoviridis | |
3,4-dihydroxybenzoate | - |
Streptomyces griseoviridis | |
ascorbate | the inhibition may result from competition for binding at the 2-oxoacid binding site between 2-oxoglutarate and L-ascorbate | Streptomyces griseoviridis | |
Co2+ | complete inhibition | Streptomyces griseoviridis | |
Cu2+ | causes a 20-30% fall in activity | Streptomyces griseoviridis | |
diethyl dicarbonate | 98% inhibition at 1 mM | Streptomyces griseoviridis | |
Fe2+ | inhibitory at a concentration higher than 0.5 mM | Streptomyces griseoviridis | |
Hg2+ | causes a 20-30% fall in activity | Streptomyces griseoviridis | |
Mn2+ | causes a 20-30% fall in activity | Streptomyces griseoviridis | |
oxalylglycine | - |
Streptomyces griseoviridis | |
Pyridine 2,4-dicarboxylate | - |
Streptomyces griseoviridis | |
Pyridine 2,5-dicarboxylate | - |
Streptomyces griseoviridis | |
Zn2+ | complete inhibition | Streptomyces griseoviridis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
2-oxoglutarate | - |
Streptomyces griseoviridis | |
0.445 | - |
L-proline | - |
Streptomyces griseoviridis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the maximal rate of hydroxylation is attained at a ferrous ion concentration of approx. 0.03 mM and proceedes at approx. 8% of the maximal rate in the absence of exogenous iron, further suggesting the presence of residual enzyme-bound iron | Streptomyces griseoviridis | |
Mn2+ | no activation, causes a 20-30% fall in activity | Streptomyces griseoviridis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
1 * 35000, SDS-PAGE | Streptomyces griseoviridis |
37700 | - |
gel filtration | Streptomyces griseoviridis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseoviridis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using chromatography on DEAE-Sepharose Fast flow column, ammonium sulfate precipitation, chromatography on phenyl-Superose column, second ammonium sulfate precipitation and column chromatography on Superdex G75 HR | Streptomyces griseoviridis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-proline + 2-oxoglutarate + O2 | 2-oxoglutarate is essential for hydroxylation | Streptomyces griseoviridis | 4-hydroxyproline + succinate + CO2 | - |
? | |
L-proline + 2-oxoglutarate + O2 | 2-oxoglutarate is essential for hydroxylation | Streptomyces griseoviridis P8648 | 4-hydroxyproline + succinate + CO2 | - |
? | |
additional information | 2-oxoglutarate cannot be replaced by oxalylglycine, 2-oxopentanoate, 2-oxoadipate, pyruvate or 2-oxomalonate | Streptomyces griseoviridis | ? | - |
? | |
additional information | 2-oxoglutarate cannot be replaced by oxalylglycine, 2-oxopentanoate, 2-oxoadipate, pyruvate or 2-oxomalonate | Streptomyces griseoviridis P8648 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, SDS-PAGE | Streptomyces griseoviridis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | - |
assay at | Streptomyces griseoviridis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
oxalylglycine | - |
Streptomyces griseoviridis | |
0.005 | - |
Pyridine 2,4-dicarboxylate | - |
Streptomyces griseoviridis | |
0.032 | - |
3,4-dihydroxybenzoate | - |
Streptomyces griseoviridis | |
0.049 | - |
Pyridine 2,5-dicarboxylate | - |
Streptomyces griseoviridis |