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Literature summary for 1.14.11.2 extracted from

  • Lawrence, C.C.; Sobey, W.J.; Field, R.A.; Baldwin, J.E.; Schofield, C.J.
    Purification and initial characterization of proline 4-hydroxylase from Streptomyces griseoviridis P8648: a 2-oxoacid, ferrous-dependent dioxygenase involved in etamycin biosynthesis (1996), Biochem. J., 313, 185-191.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
catalase activation Streptomyces griseoviridis
pyridine 2,6-dicarboxylate activator Streptomyces griseoviridis

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate at concentrations higher than 0.5 mM, decreases activity Streptomyces griseoviridis
3,4-dihydroxybenzoate
-
Streptomyces griseoviridis
ascorbate the inhibition may result from competition for binding at the 2-oxoacid binding site between 2-oxoglutarate and L-ascorbate Streptomyces griseoviridis
Co2+ complete inhibition Streptomyces griseoviridis
Cu2+ causes a 20-30% fall in activity Streptomyces griseoviridis
diethyl dicarbonate 98% inhibition at 1 mM Streptomyces griseoviridis
Fe2+ inhibitory at a concentration higher than 0.5 mM Streptomyces griseoviridis
Hg2+ causes a 20-30% fall in activity Streptomyces griseoviridis
Mn2+ causes a 20-30% fall in activity Streptomyces griseoviridis
oxalylglycine
-
Streptomyces griseoviridis
Pyridine 2,4-dicarboxylate
-
Streptomyces griseoviridis
Pyridine 2,5-dicarboxylate
-
Streptomyces griseoviridis
Zn2+ complete inhibition Streptomyces griseoviridis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
2-oxoglutarate
-
Streptomyces griseoviridis
0.445
-
L-proline
-
Streptomyces griseoviridis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the maximal rate of hydroxylation is attained at a ferrous ion concentration of approx. 0.03 mM and proceedes at approx. 8% of the maximal rate in the absence of exogenous iron, further suggesting the presence of residual enzyme-bound iron Streptomyces griseoviridis
Mn2+ no activation, causes a 20-30% fall in activity Streptomyces griseoviridis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
1 * 35000, SDS-PAGE Streptomyces griseoviridis
37700
-
gel filtration Streptomyces griseoviridis

Organism

Organism UniProt Comment Textmining
Streptomyces griseoviridis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using chromatography on DEAE-Sepharose Fast flow column, ammonium sulfate precipitation, chromatography on phenyl-Superose column, second ammonium sulfate precipitation and column chromatography on Superdex G75 HR Streptomyces griseoviridis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-proline + 2-oxoglutarate + O2 2-oxoglutarate is essential for hydroxylation Streptomyces griseoviridis 4-hydroxyproline + succinate + CO2
-
?
L-proline + 2-oxoglutarate + O2 2-oxoglutarate is essential for hydroxylation Streptomyces griseoviridis P8648 4-hydroxyproline + succinate + CO2
-
?
additional information 2-oxoglutarate cannot be replaced by oxalylglycine, 2-oxopentanoate, 2-oxoadipate, pyruvate or 2-oxomalonate Streptomyces griseoviridis ?
-
?
additional information 2-oxoglutarate cannot be replaced by oxalylglycine, 2-oxopentanoate, 2-oxoadipate, pyruvate or 2-oxomalonate Streptomyces griseoviridis P8648 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 35000, SDS-PAGE Streptomyces griseoviridis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
26
-
assay at Streptomyces griseoviridis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0004
-
oxalylglycine
-
Streptomyces griseoviridis
0.005
-
Pyridine 2,4-dicarboxylate
-
Streptomyces griseoviridis
0.032
-
3,4-dihydroxybenzoate
-
Streptomyces griseoviridis
0.049
-
Pyridine 2,5-dicarboxylate
-
Streptomyces griseoviridis