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Literature summary for 1.14.11.2 extracted from
- Purification and partial characterization of the enzyme for the hydroxylation of proline in protocollagen (1967), Arch. Biochem. Biophys., 118, 611-618.
No PubMed abstract available
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | subcellular distribution, differential centrifugation | Gallus gallus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Gallus gallus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 200000 | - |
gel filtration | Gallus gallus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using ammonium sulfate fractionation, fractionation with calcium phosphate gel, fractionation with alumina gel, chromatography on DEAE-Sephadex and polyacrylamide gel filtration | Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Gallus gallus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (L-prolylglycyl-L-prolyl)n + 2-oxoglutarate + O2 | molecular weight of the peptide substrate: about 4000 | Gallus gallus | 4-hydroxyproline containing peptide | - |
? | |
| additional information | the enzyme is specific for proline in the second position after glycine, the position in which the hydroxyproline in collagen is found. No reaction with: free proline, glycyl-L-prolyl-L-proline or poly-L-proline II with a molecular weight of about 15000 | Gallus gallus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Gallus gallus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | - |
Gallus gallus | |
| ascorbate | - |
Gallus gallus | |
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Release 2023.1 (January 2023)
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