Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.11.2 extracted from

  • Myllylä, R.; Tuderman, L.; Kivirikko, K.I.
    Mechanism of the prolyl hydroxylase reaction. 2. Kinetic analysis of the reaction sequence (1977), Eur. J. Biochem., 80, 349-357.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activation Gallus gallus
catalase activation Gallus gallus
dithiothreitol activation Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
CO2 5% inhibition at 3.6 mM, 35% inhibition at 7.2 mM and 75% inhibition at 12 mM Gallus gallus
Collagen product inhibitor, noncompetitive with respect to all substrates of the reaction Gallus gallus
poly(L-proline) competitive inhibitor with respect to the polypeptide substrate and uncompetitive with respect to Fe2+ and 2-oxoglutarate Gallus gallus
succinate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Gallus gallus

Reaction

Reaction Comment Organism Reaction ID
procollagen L-proline + 2-oxoglutarate + O2 = procollagen trans-4-hydroxy-L-proline + succinate + CO2 mechanism, kinetic analysis of the reaction sequence Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Gallus gallus

Cofactor

Cofactor Comment Organism Structure
ascorbate
-
Gallus gallus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4 1.6 succinate calculated from the slopes or intercepts Gallus gallus
2.3 2.5 poly(L-proline) calculated from the slopes or intercepts Gallus gallus
40
-
Collagen from basement membranes, calculated from the slopes or intercepts Gallus gallus
170 180 Collagen from skin, calculated from the slopes or intercepts Gallus gallus