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Literature summary for 1.14.11.2 extracted from
- The 2-oxoglutarate binding site of prolyl 4-hydroxylase. Identification of distinct subsites and evidence for 2-oxoglutarate decarboxylation in a ligand reaction at the enzyme-bound ferrous ion (1984), Eur. J. Biochem., 138, 239-245.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | activation | Gallus gallus | |
| catalase | activation | Gallus gallus | |
| dithiothreitol | activation | Gallus gallus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoadipinate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| 2-oxobutyrate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| 2-oxosuccinate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+; i.e. oxaloacetate | Gallus gallus | |
| 2-oxovalerate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| 3-oxoglutarate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| adipinate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| benzene 1,2-dicarboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| Benzene 1,3-dicarboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| Benzene 1,4-dicarboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| benzoate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| Glutarate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| levulinate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| malonate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| pyridine 2,3-dicarboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| Pyridine 2,4-dicarboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| Pyridine 2,5-dicarboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| pyridine 2,6-dicarboxylate | competitive with respect to Fe2+ and noncompetitive with respect to 2-oxoglutarate | Gallus gallus | |
| pyridine 2-carboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| Pyridine 3,4-dicarboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| Pyridine 3,5-dicarboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| pyridine 3-carboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| Pyridine 4-carboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| pyruvate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
| succinate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
carboxymethylated collagen L-proline | as cosubstrate | Gallus gallus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Gallus gallus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Gallus gallus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (Pro-Pro-Gly)n + 2-oxoglutarate + O2 | n: 1,5,10 | Gallus gallus | (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 | n: 1,5,10 | ? | |
| carboxymethylated collagen L-proline + 2-oxoglutarate + O2 | - |
Gallus gallus | carboxymethylated collagen-trans-4-hydroxy-L-proline + succinate + CO2 | - |
r | |
| additional information | thermal denaturing of the triple-helical conformation of the substrate before hydroxylation | Gallus gallus | ? | - |
? | |
| additional information | characterization of the 2-oxoglutarate binding site of the enzyme, 2-oxosuccinate, 2-oxovalerate, 2-oxobutyrate, 3-oxoglutarate or pyridine 2,5-dicarboxylate do not replace 2-oxoglutarate as cosubstrates | Gallus gallus | ? | - |
? | |
| protocollagen + 2-oxoglutarate + O2 | 2-oxoadipate can replace 2-oxoglutarate as cosubstrate | Gallus gallus | 4-hydroxyproline containing protocollagen + succinate + CO2 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Gallus gallus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | - |
Gallus gallus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0008 | - |
Pyridine-2,5-dicarboxylate | - |
Gallus gallus | |
| 0.002 | - |
Pyridine-2,4-dicarboxylate | - |
Gallus gallus | |
| 0.025 | - |
pyridine 2-carboxylate | - |
Gallus gallus | |
| 0.1 | - |
2-oxosuccinate | - |
Gallus gallus | |
| 0.21 | - |
pyridine 2,3-dicarboxylate | - |
Gallus gallus | |
| 0.4 | - |
succinate | - |
Gallus gallus | |
| 0.5 | - |
pyridine 3-carboxylate | - |
Gallus gallus | |
| 0.8 | - |
3-oxoglutarate | - |
Gallus gallus | |
| 0.9 | - |
2-oxoadipinate | - |
Gallus gallus | |
| 0.9 | - |
Pyridine 3,5-dicarboxylate | - |
Gallus gallus | |
| 0.9 | - |
Pyridine 4-carboxylate | - |
Gallus gallus | |
| 2.2 | - |
Pyridine 3,4-dicarboxylate | - |
Gallus gallus | |
| 3.3 | - |
Benzene 1,4-dicarboxylate | - |
Gallus gallus | |
| 4.2 | - |
Glutarate | - |
Gallus gallus | |
| 5.8 | - |
Benzene 1,3-dicarboxylate | - |
Gallus gallus | |
| 5.9 | - |
malonate | - |
Gallus gallus | |
| 7.6 | - |
2-oxobutyrate | - |
Gallus gallus | |
| 9.4 | - |
2-oxovalerate | - |
Gallus gallus | |
| 9.9 | - |
benzoate | - |
Gallus gallus | |
| 10 | - |
benzene 1,2-dicarboxylate | value above | Gallus gallus | |
| 15 | - |
pyruvate | - |
Gallus gallus | |
| 15 | - |
levulinate | value above | Gallus gallus | |
| 15 | - |
adipinate | value above | Gallus gallus | |
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