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Literature summary for 1.14.11.2 extracted from

  • De Jong, L.; Kemp, A.
    Stoicheiometry and kinetics of the prolyl 4-hydroxylase partial reaction (1984), Biochim. Biophys. Acta, 787, 105-111.
    View publication on PubMed
Search for more literature for 1.14.11.2

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activation Gallus gallus
catalase activation Gallus gallus
dithiothreitol activation Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
Pyridine 2,4-dicarboxylate
-
 Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 2-oxoglutarate partial reaction or complete reaction with in the presence of (Pro-Pro-Gly)n as a peptide substrate Gallus gallus
0.19
-
 O2 complete reaction with (Pro-Pro-Gly)5 as a peptide substrate Gallus gallus
1.5
-
 O2 partial reaction Gallus gallus
1.8
-
 (L-Pro-L-Pro-Gly)5 O2 with cosubstrate polyprolin Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(L-Pro-L-Pro-Gly)5 + 2-oxoglutarate + O2
-
 Gallus gallus (L-Pro-L-Pro-Gly)5-trans-4-hydroxy-L-proline + succinate + CO2
-
 r
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
2-oxoglutarate + O2 + ascorbate uncoupled oxidative decarboxylation Gallus gallus succinate + dehydroascorbate + CO2 + H2O
-
 ?
additional information in the absence of a peptidylproline substrate, the oxidative decarboxylation of 2-oxoglutarate by the enzyme is stoichiometrically coupled to the oxidation of ascorbate Gallus gallus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 assay at Gallus gallus

Cofactor

Cofactor Comment Organism Structure
ascorbate
-
 Gallus gallus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.002
-
 Pyridine 2,4-dicarboxylate
-
 Gallus gallus