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Literature summary for 1.14.11.2 extracted from

  • Gunzler, V.; Hanauske-Abel, H.M.; Myllylä, R.; Mohr, J.; Kivirikko, K.I.
    Time-dependent inactivation of chick-embryo prolyl 4-hydroxylase by coumalic acid. Evidence for a syncatalytic mechanism (1987), Biochem. J., 242, 163-169.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.2

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activation Gallus gallus
bovine serum albumin activation Homo sapiens
catalase activation Gallus gallus
catalase activation Homo sapiens
dithiothreitol activation Gallus gallus
dithiothreitol activation Homo sapiens

General Stability

General Stability Organism
unstable in absence of 2-oxoglutarate and either Fe2+ or ascorbate Gallus gallus
unstable in absence of 2-oxoglutarate and either Fe2+ or ascorbate Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3,4-dihydroxybenzoate
-
 Gallus gallus
3,4-dihydroxybenzoate
-
 Homo sapiens
4-hydroxybenzoate
-
 Gallus gallus
4-hydroxybenzoate
-
 Homo sapiens
Coumalic acid competitive inhibitor, potential syncatalytic inhibitor, time-dependent inactivation, increasing concentrations of Fe2+ enhance the inactivation; i.e. 2-oxo-1,2H-pyran-5-carboxylic acid Gallus gallus
Coumalic acid competitive inhibitor, potential syncatalytic inhibitor, time-dependent inactivation, increasing concentrations of Fe2+ enhance the inactivation; i.e. 2-oxo-1,2H-pyran-5-carboxylic acid Homo sapiens
Pyridine 2,4-dicarboxylate
-
 Gallus gallus
Pyridine 2,4-dicarboxylate
-
 Homo sapiens
Pyridine 2,5-dicarboxylate
-
 Gallus gallus
Pyridine 2,5-dicarboxylate
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 2-oxoglutarate
-
 Gallus gallus
0.022
-
 2-oxoglutarate
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate fractionation, affinity chromatography on poly(L-proline) coupled to Sepharose 4B, DEAE-cellulose chromatography, and gel filtration Gallus gallus
using ammonium sulfate fractionation, affinity chromatography on poly(L-proline) coupled to Sepharose 4B, DEAE-cellulose chromatography, and gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Homo sapiens (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?

Subunits

Subunits Comment Organism
tetramer the 2-oxoglutarate-binding site of the enzyme is located within the alpha-subunit Gallus gallus
tetramer the 2-oxoglutarate-binding site of the enzyme is located within the alpha-subunit Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Gallus gallus
37
-
 assay at Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
 increased spontaneous inactivation at pH values above in the absence of an inactivating compound Gallus gallus
7.5
-
 increased spontaneous inactivation at pH values above in the absence of an inactivating compound Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ascorbate
-
 Gallus gallus
ascorbate
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0008
-
 Pyridine-2,5-dicarboxylate
-
 Gallus gallus
0.0008
-
 Pyridine-2,5-dicarboxylate
-
 Homo sapiens
0.002
-
 Pyridine-2,4-dicarboxylate
-
 Gallus gallus
0.002
-
 Pyridine-2,4-dicarboxylate
-
 Homo sapiens
0.005
-
 3,4-dihydroxybenzoate
-
 Gallus gallus
0.005
-
 3,4-dihydroxybenzoate
-
 Homo sapiens
3.2
-
 4-hydroxybenzoate
-
 Gallus gallus
3.2
-
 4-hydroxybenzoate
-
 Homo sapiens
17
-
 Coumalic acid S-parabolic competitive inhibition with respect to 2-oxoglutarate Gallus gallus
17
-
 Coumalic acid S-parabolic competitive inhibition with respect to 2-oxoglutarate Homo sapiens