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Literature summary for 1.14.11.17 extracted from

  • Henderson, K.L.; Mueller, T.A.; Hausinger, R.P.; Emerson, J.P.
    Calorimetric assessment of Fe(2+) binding to ?-ketoglutarate/taurine dioxygenase ironing out the energetics of metal coordination by the 2-His-1-carboxylate facial triad (2015), Inorg. Chem., 54, 2278-2283.
    View publication on PubMed
Search for more literature for 1.14.11.17

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli BL21(DE3) cells harboring plasmid pME4141 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the thermodynamic properties of Fe2+ binding to the 2-His-1-carboxylate facial triad in alpha-ketoglutarate/taurine dioxygenase (TauD) are explored using isothermal titration calorimetry. Direct titrations of Fe2+ into TauD and chelation experiments involving the titration of thylenediaminetetraacetic acid into Fe2+-TauD are performed under an anaerobic environment to yield a binding equilibrium of 2400000 (Kd = 43 nM) and a DELTAG(0) value of -10.1 kcal/mol. Further analysis of the enthalpy/entropy contributions indicates a highly enthalpic binding event, where DELTAH = -11.6 kcal/mol. Investigations into the unfavorable entropy term leads to the observation of water molecules becoming organized within the Fe2+-TauD structure Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P37610
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
taurine + 2-oxoglutarate + O2
-
 Escherichia coli sulfite + aminoacetaldehyde + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
alpha-ketoglutarate/taurine dioxygenase
-
 Escherichia coli
TauD
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli