Cloned (Comment) | Organism |
---|---|
the anti gibberellin 24 single monoclonal antibody gene (anti-GA24 scFv) is PCR-amplified with plasmid pH1L24B and introduced into the binary vector pBI-ER-D, transferred into Agrobacterium tumefaciens strain LBA4404 to transform Arabidospsis thaliana, the antibody is fused with the green fluorescence protein (GFP), a c-myc epitope tag and KDEL ER-retention signal | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | transgenic plant lines with green fluorescence protein-anti gibberellin24 antibody fusion construct (GFP-scFv), called A24-1, 2, and 3, up-regulation in the transgenic plant line A24-2 enhances biosynthesis of gibberellin 24 which accumulates compared to wild-type. GFP-scFv reduces rosette leaf development, delays flower induction, reduces stem elongation of main culm, especially in the early stage of inflorescence growth, later stem elongation of the main culm and lateral shoot elongation is much less affected | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
rosette leaves are frozen in liquid nitrogen and ground to a fine powder, protein extracted with 50 mM Tris-HCl, pH 8.0, containing 200 mM NaCl, 5 mM EDTA, 0.1% Tween 20 and a proteinase inhibitor mixture, SDS-PAGE | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epidermal cell | - |
Arabidopsis thaliana | - |
rosette | - |
Arabidopsis thaliana | - |
Synonyms | Comment | Organism |
---|---|---|
AtGA3ox1 | - |
Arabidopsis thaliana |
GA3 oxidase | - |
Arabidopsis thaliana |
gibberellin 3 oxidase | - |
Arabidopsis thaliana |