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Literature summary for 1.14.11.15 extracted from

  • Urakami, E.; Yamaguchi, I.; Asami, T.; Conrad, U.; Suzuki, Y.
    Immunomodulation of gibberellin biosynthesis using an anti-precursor gibberellin antibody confers gibberellin-deficient phenotypes (2008), Planta, 228, 863-873.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the anti gibberellin 24 single monoclonal antibody gene (anti-GA24 scFv) is PCR-amplified with plasmid pH1L24B and introduced into the binary vector pBI-ER-D, transferred into Agrobacterium tumefaciens strain LBA4404 to transform Arabidospsis thaliana, the antibody is fused with the green fluorescence protein (GFP), a c-myc epitope tag and KDEL ER-retention signal Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information transgenic plant lines with green fluorescence protein-anti gibberellin24 antibody fusion construct (GFP-scFv), called A24-1, 2, and 3, up-regulation in the transgenic plant line A24-2 enhances biosynthesis of gibberellin 24 which accumulates compared to wild-type. GFP-scFv reduces rosette leaf development, delays flower induction, reduces stem elongation of main culm, especially in the early stage of inflorescence growth, later stem elongation of the main culm and lateral shoot elongation is much less affected Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
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-
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Purification (Commentary)

Purification (Comment) Organism
rosette leaves are frozen in liquid nitrogen and ground to a fine powder, protein extracted with 50 mM Tris-HCl, pH 8.0, containing 200 mM NaCl, 5 mM EDTA, 0.1% Tween 20 and a proteinase inhibitor mixture, SDS-PAGE Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
epidermal cell
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Arabidopsis thaliana
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rosette
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Arabidopsis thaliana
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Synonyms

Synonyms Comment Organism
AtGA3ox1
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Arabidopsis thaliana
GA3 oxidase
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Arabidopsis thaliana
gibberellin 3 oxidase
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Arabidopsis thaliana