Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | 1 mM and more, 1.7fold stimulation | Cucurbita maxima | |
DTT | stimulates. Stimulation is negated when added together with DTT | Cucurbita maxima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0087 | - |
gibberellin a15 | - |
Cucurbita maxima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | stimulates. Stimulation is negated when added together with DTT. If Fe2+ is omitted, the activities are reduced by 25% compared with the maximal value reached at 0.008-0.03 mM FeSO4 | Cucurbita maxima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
gel filtration | Cucurbita maxima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
gibberellin a20 + 2-oxoglutarate + O2 | Cucurbita maxima | involved in biosynthesis of gibberellin | gibberellin a1 + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cucurbita maxima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Cucurbita maxima |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | - |
Cucurbita maxima | - |
seed | endosperm | Cucurbita maxima | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.065 | - |
- |
Cucurbita maxima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gibberellin a15 + 2-oxoglutarate + O2 | - |
Cucurbita maxima | gibberellin a37 + succinate + CO2 | - |
? | |
gibberellin a20 + 2-oxoglutarate + O2 | - |
Cucurbita maxima | gibberellin a1 + succinate + CO2 | - |
? | |
gibberellin a20 + 2-oxoglutarate + O2 | involved in biosynthesis of gibberellin | Cucurbita maxima | gibberellin a1 + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000-50000, SDS-PAGE | Cucurbita maxima |