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Literature summary for 1.14.11.15 extracted from

  • Lange, T.; Schweimer, A.; Ward, D.A.; Hedden, P.; Graebe, J.E.
    Separation and characterization of three 2-oxoglutarate-dependent dioxygenases from Cucurbita maxima L. endosperm involved in gibberellin biosynthesis (1994), Planta, 195, 98-107.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
ascorbate 1 mM and more, 1.7fold stimulation Cucurbita maxima
DTT stimulates. Stimulation is negated when added together with DTT Cucurbita maxima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0087
-
gibberellin a15
-
Cucurbita maxima

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ stimulates. Stimulation is negated when added together with DTT. If Fe2+ is omitted, the activities are reduced by 25% compared with the maximal value reached at 0.008-0.03 mM FeSO4 Cucurbita maxima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
gel filtration Cucurbita maxima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
gibberellin a20 + 2-oxoglutarate + O2 Cucurbita maxima involved in biosynthesis of gibberellin gibberellin a1 + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Cucurbita maxima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Cucurbita maxima

Source Tissue

Source Tissue Comment Organism Textmining
endosperm
-
Cucurbita maxima
-
seed endosperm Cucurbita maxima
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.065
-
-
Cucurbita maxima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gibberellin a15 + 2-oxoglutarate + O2
-
Cucurbita maxima gibberellin a37 + succinate + CO2
-
?
gibberellin a20 + 2-oxoglutarate + O2
-
Cucurbita maxima gibberellin a1 + succinate + CO2
-
?
gibberellin a20 + 2-oxoglutarate + O2 involved in biosynthesis of gibberellin Cucurbita maxima gibberellin a1 + succinate + CO2
-
?

Subunits

Subunits Comment Organism
? x * 40000-50000, SDS-PAGE Cucurbita maxima