Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | 2 mg/ml, 30% activation of purified enzyme, stimulation by bovine serum albumin and catalase is additive and less pronounced in crude cell extract | Phaseolus vulgaris | |
catalase | 0.5 mg/ml, 30% activation of purified enzyme, stimulation by catalase and bovine serum albumin is additive and less pronounced in crude cell extract | Phaseolus vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-bipyridyl | - |
Phaseolus vulgaris | |
Cd2+ | 0.5 mM, strong | Phaseolus vulgaris | |
Co2+ | 0.5 mM, strong | Phaseolus vulgaris | |
Cu2+ | 0.5 mM, strong | Phaseolus vulgaris | |
gibberellin a15 | inhibits 3beta-hydroxylation of gibberellin a20 | Phaseolus vulgaris | |
gibberellin a20 | - |
Phaseolus vulgaris | |
gibberellin a44 | inhibits 3beta-hydroxylation of gibberellin a20 | Phaseolus vulgaris | |
gibberellin a5 | inhibits 3beta-hydroxylation of gibberellin a20 | Phaseolus vulgaris | |
gibberellin a9 | inhibits 3beta-hydroxylation of gibberellin a20 | Phaseolus vulgaris | |
Hg2+ | 0.5 mM, strong | Phaseolus vulgaris | |
Mn2+ | 0.5 mM, strong | Phaseolus vulgaris | |
Ni2+ | 0.5 mM, strong | Phaseolus vulgaris | |
Zn2+ | 0.5 mM, strong | Phaseolus vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00029 | - |
gibberellin a20 | - |
Phaseolus vulgaris | |
0.00033 | - |
gibberellin a9 | - |
Phaseolus vulgaris | |
0.25 | - |
2-oxoglutarate | with gibberellin a20 as cosubstrate | Phaseolus vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | activates, at least 0.2 mM for full activation | Phaseolus vulgaris | |
Fe3+ | activation in the presence of ascorbate | Phaseolus vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
gel filtration | Phaseolus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaseolus vulgaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Phaseolus vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | immature | Phaseolus vulgaris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Phaseolus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gibberellin a20 + 2-oxoglutarate + O2 | - |
Phaseolus vulgaris | gibberellin a1 + succinate + CO2 | - |
? | |
gibberellin a9 + 2-oxoglutarate + O2 | - |
Phaseolus vulgaris | gibberellin a4 + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42000, SDS-PAGE | Phaseolus vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
- |
Phaseolus vulgaris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | about half-maximal activity at pH 7.0 and pH 9.0 | Phaseolus vulgaris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | 5 mM, 10fold stimulation | Phaseolus vulgaris |