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Literature summary for 1.14.11.13 extracted from

  • Park, S.H.; Nakajima, M.; Hasegawa, M.; Yamaguchi, I.
    Similarities and differences between the characteristics of gibberellin-binding protein and gibberellin 2-oxidases in adzuki bean (Vigna angularis) seedlings (2005), Biosci. Biotechnol. Biochem., 69, 1508-1514.
    View publication on PubMed
Search for more literature for 1.14.11.13

Activating Compound

Activating Compound Comment Organism Structure
2-oxoglutarate
-
 Vigna angularis
additional information the combination of 1 mM 2-oxoglutarate and 4 mM ascorbate increases activity Vigna angularis

Inhibitors

Inhibitors Comment Organism Structure
gibberellin 1 50% inhibition at 2-10 nmol Vigna angularis
gibberellin 3 50% inhibition at 2-10 nmol Vigna angularis
gibberellin 4 50% inhibition at 0.2-0.3 nmol Vigna angularis
gibberellin 9 50% inhibition at 11 nmol Vigna angularis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM, activating effect in combination with 2-oxoglutarate Vigna angularis
Fe2+ 1 mM, increases activity, in combination with 2-oxoglutarate Vigna angularis

Organism

Organism UniProt Comment Textmining
Vigna angularis
-
 cultivar Dainagon
-

Purification (Commentary)

Purification (Comment) Organism
Sephadex G-25 column chromatography and Superdex G-25 column chromatography, Mini S column chromatography and gel filtration chromatography Vigna angularis

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Vigna angularis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gibberellin 1 + 2-oxoglutarate + O2
-
 Vigna angularis 2beta-hydroxygibberellin 1 + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
gibberellin 2-oxidase
-
 Vigna angularis

pI Value

Organism Comment pI Value Maximum pI Value
Vigna angularis isoelectrofocusing 9 7.5