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Literature summary for 1.14.11.11 extracted from

  • Vakili, B.; Karimi, F.; Sharifi, M.; Behmanesh, M.
    Chromium-induced tropane alkaloid production and H6H gene expression in Atropa belladonna L. (Solanaceae) in vitro-propagated plantlets (2012), Plant Physiol. Biochem., 52, 98-103.
    View publication on PubMed
Search for more literature for 1.14.11.11

Activating Compound

Activating Compound Comment Organism Structure
ascorbate required for activity Atropa belladonna

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for activity Atropa belladonna

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-hyoscyamine + 2-oxoglutarate + O2 Atropa belladonna
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 (6S)-hydroxyhyoscyamine + succinate + CO2
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 ?

Organism

Organism UniProt Comment Textmining
Atropa belladonna Q9XJ43 collected from Sari-Garmestan of Mazandaran province in the North of Iran, gene h6h
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Source Tissue

Source Tissue Comment Organism Textmining
root
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 Atropa belladonna
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shoot
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 Atropa belladonna
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-hyoscyamine + 2-oxoglutarate + O2
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 Atropa belladonna (6S)-hydroxyhyoscyamine + succinate + CO2
-
 ?
additional information the enzyme also catalyzes the reaction of EC 1.14.11.14 Atropa belladonna ?
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 ?

Synonyms

Synonyms Comment Organism
H6H
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 Atropa belladonna
hyoscyamine 6beta-hydroxylase
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 Atropa belladonna

Expression

Organism Comment Expression
Atropa belladonna trivalent chromium, as KCr(SO4)2, induces the enzyme expression at 1 mM up

General Information

General Information Comment Organism
evolution the enzyme is a member of the 2-oxoglutarate-dependent dioxygenase family Atropa belladonna
metabolism hyoscyamine 6b-hydroxylase catalyzes two consecutive oxidation reactions. The first reaction is the hydroxylation of hyoscyamine to 6beta-hydroxyhyoscyamine and the second is epoxidation of 6beta-hydroxyhyoscyamine yielding scopolamine, EC 1.14.11.14, the final metabolite in the tropane alkaloid biosynthetic pathway Atropa belladonna
additional information the transcript level of H6H increased under chromium treatment. This treatment also increases hyoscyamine and scopolamine contents, phenotype with decreased the growth parameters (weights and lengths of the plantlets) and chlorophyll contents and increased proline contents, overview Atropa belladonna