Literature summary for 1.14.11.11 extracted from

Matsuda, J.; Hashimoto, T.; Yamada, Y.
Analysis of active-site residues in hyoscyamine 6beta-hydroxylase (1997), Plant Biotechnol., 14, 51-57.

No PubMed abstract available

Search for more literature for 1.14.11.11

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli as a fusion protein to maltose-binding protein	Hyoscyamus niger

Protein Variants

Protein Variants	Comment	Organism
D219H	inactive mutant enzyme	Hyoscyamus niger
D219N	inactive mutant enzyme	Hyoscyamus niger
H217Q	inactive mutant enzyme	Hyoscyamus niger
H66Q	mutant enzyme has 97% of the activity of the wild-type enzyme	Hyoscyamus niger
S274Q	inactive mutant enzyme	Hyoscyamus niger

Organism

Organism	UniProt	Comment	Textmining
Hyoscyamus niger	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-hyoscyamine + 2-oxoglutarate + O2	-	Hyoscyamus niger	6beta-hydroxyhyoscyamine + succinate + CO2	-	?

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Release 2023.1 (January 2023)