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Literature summary for 1.14.11.11 extracted from

  • Hashimoto, T.; Yamada, Y.
    Hyoscyamine 6beta-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, an alkaloid-producing root cultures (1986), Plant Physiol., 81, 619-625.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.11

Activating Compound

Activating Compound Comment Organism Structure
acetone 10% v/v, activation Atropa belladonna
acetone 10% v/v, activation Duboisia leichhardtii
acetone 10% v/v, activation Hyoscyamus niger
acetone 10% v/v, activation Hyoscyamus muticus
acetone 10% v/v, activation Hyoscyamus pusillus
acetone 10% v/v, activation Hyoscyamus albus
acetone 10% v/v, activation Datura metel
acetone 10% v/v, activation Hyoscyamus gyorffi
ascorbate required Atropa belladonna
ascorbate required Duboisia leichhardtii
ascorbate required Hyoscyamus niger
ascorbate required Hyoscyamus muticus
ascorbate required Hyoscyamus pusillus
ascorbate required Hyoscyamus albus
ascorbate required Datura metel
ascorbate required Hyoscyamus gyorffi
catalase activation Atropa belladonna
catalase activation Duboisia leichhardtii
catalase activation Hyoscyamus niger
catalase activation Hyoscyamus muticus
catalase activation Hyoscyamus pusillus
catalase activation Hyoscyamus albus
catalase activation Datura metel
catalase activation Hyoscyamus gyorffi
dithiothreitol activation Atropa belladonna
dithiothreitol activation Duboisia leichhardtii
dithiothreitol activation Hyoscyamus niger
dithiothreitol activation Hyoscyamus muticus
dithiothreitol activation Hyoscyamus pusillus
dithiothreitol activation Hyoscyamus albus
dithiothreitol activation Datura metel
dithiothreitol activation Hyoscyamus gyorffi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 L-hyoscyamine
-
 Hyoscyamus niger
0.057
-
 2-oxoglutarate
-
 Hyoscyamus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Atropa belladonna
Fe2+ required Duboisia leichhardtii
Fe2+ required Hyoscyamus niger
Fe2+ required Hyoscyamus muticus
Fe2+ required Hyoscyamus pusillus
Fe2+ required Hyoscyamus albus
Fe2+ required Datura metel
Fe2+ required Hyoscyamus gyorffi

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 10% v/v, activation Atropa belladonna
Acetone 10% v/v, activation Duboisia leichhardtii
Acetone 10% v/v, activation Hyoscyamus niger
Acetone 10% v/v, activation Hyoscyamus muticus
Acetone 10% v/v, activation Hyoscyamus pusillus
Acetone 10% v/v, activation Hyoscyamus albus
Acetone 10% v/v, activation Datura metel
Acetone 10% v/v, activation Hyoscyamus gyorffi

Organism

Organism UniProt Comment Textmining
Atropa belladonna
-
-
-
Datura metel
-
-
-
Duboisia leichhardtii
-
-
-
Hyoscyamus albus
-
-
-
Hyoscyamus gyorffi
-
-
-
Hyoscyamus muticus
-
-
-
Hyoscyamus niger
-
-
-
Hyoscyamus pusillus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Hyoscyamus niger

Source Tissue

Source Tissue Comment Organism Textmining
root culture Atropa belladonna
-
root culture Duboisia leichhardtii
-
root culture Hyoscyamus niger
-
root culture Hyoscyamus muticus
-
root culture Hyoscyamus pusillus
-
root culture Hyoscyamus albus
-
root culture Datura metel
-
root culture Hyoscyamus gyorffi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-hyoscyamine + 2-oxoglutarate + O2
-
 Atropa belladonna 6beta-hydroxyhyoscyamine + succinate + CO2
-
 ?
L-hyoscyamine + 2-oxoglutarate + O2
-
 Duboisia leichhardtii 6beta-hydroxyhyoscyamine + succinate + CO2
-
 ?
L-hyoscyamine + 2-oxoglutarate + O2
-
 Hyoscyamus muticus 6beta-hydroxyhyoscyamine + succinate + CO2
-
 ?
L-hyoscyamine + 2-oxoglutarate + O2
-
 Hyoscyamus pusillus 6beta-hydroxyhyoscyamine + succinate + CO2
-
 ?
L-hyoscyamine + 2-oxoglutarate + O2
-
 Hyoscyamus albus 6beta-hydroxyhyoscyamine + succinate + CO2
-
 ?
L-hyoscyamine + 2-oxoglutarate + O2
-
 Datura metel 6beta-hydroxyhyoscyamine + succinate + CO2
-
 ?
L-hyoscyamine + 2-oxoglutarate + O2
-
 Hyoscyamus gyorffi 6beta-hydroxyhyoscyamine + succinate + CO2
-
 ?
L-hyoscyamine + 2-oxoglutarate + O2 only the L-isomer of hyoscyamine serves as substrate, D-hyoscyamine is nearly inactive Hyoscyamus niger 6beta-hydroxyhyoscyamine + succinate + CO2
-
 ?