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Literature summary for 1.14.11.11 extracted from
- Purification and characterization of hyoscyamine 6 beta-hydroxylase from root cultures of Hyoscyamus niger L. hydroxylase and epoxidase activities in the enzyme preparation (1987), Eur. J. Biochem., 164, 277-285.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 5,7-dimethyl-5,6,7,8-tetrahydropterin | partial activation | Hyoscyamus niger |  |
| ascorbate | 4 mM, 5.9fold stimulation | Hyoscyamus niger | |
| catalase | activation | Hyoscyamus niger | |
| dehydroascorbate | partial activation | Hyoscyamus niger | |
| dithiothreitol | partial activation | Hyoscyamus niger | |
| isoascorbate | partial activation | Hyoscyamus niger | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 0.1 mM, 35% inhibition | Hyoscyamus niger | |
| 2,2'-dipyridyl | 0.1 mM, 15% inhibition | Hyoscyamus niger | |
| 3,4-dihydroxybenzoate | - |
Hyoscyamus niger | |
| 3,4-dihydroxycinnamate | - |
Hyoscyamus niger | |
| 3,4-dihydroxymandelate | - |
Hyoscyamus niger | |
| 3,4-Dihydroxyphenylacetate | - |
Hyoscyamus niger | |
| 3,4-Dihydroxyphenylpropionate | - |
Hyoscyamus niger | |
| 3-oxoglutaric acid | - |
Hyoscyamus niger | |
| 5,7-dehydrohyoscyamine | - |
Hyoscyamus niger | |
| Apoatropine | - |
Hyoscyamus niger | |
| Bathocuproine | 0.1 mM, 27% inhibition | Hyoscyamus niger | |
| Ca2+ | 0.4 mM, 11% inhibition | Hyoscyamus niger | |
| Cd2+ | 0.4 mM, complete inhibition | Hyoscyamus niger | |
| Co2+ | 0.4 mM, complete inhibition | Hyoscyamus niger | |
| Cu2+ | 0.4 mM, complete inhibition | Hyoscyamus niger | |
| diethyldithiocarbamate | 0.1 mM, 37% inhibition | Hyoscyamus niger | |
| EDTA | 0.1 mM, 67% inhibition | Hyoscyamus niger | |
| Fe3+ | 0.4 mM, 28% inhibition | Hyoscyamus niger | |
| fumarate | - |
Hyoscyamus niger | |
| Hg2+ | 0.4 mM, 96% inhibition | Hyoscyamus niger | |
| L-Homatropine | - |
Hyoscyamus niger | |
| Mn2+ | 0.4 mM, 95% inhibition | Hyoscyamus niger | |
| Ni2+ | 0.4 mM, 96% inhibition | Hyoscyamus niger | |
| nitroblue tetrazolium | - |
Hyoscyamus niger | |
| Noratropine-N-acetic acid | - |
Hyoscyamus niger | |
| Phenylacetyltropine | - |
Hyoscyamus niger | |
| pyridine 2,3-dicarboxylate | competitive with respect to 2-oxoglutarate | Hyoscyamus niger | |
| Pyridine 2,4-dicarboxylate | competitive with respect to 2-oxoglutarate | Hyoscyamus niger | |
| Pyridine 3,4-dicarboxylate | - |
Hyoscyamus niger | |
| Tiron | 0.1 mM, 44% inhibition | Hyoscyamus niger | |
| Zn2+ | 0.4 mM, complete inhibition | Hyoscyamus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
6,7-Dehydrohyoscyamine | - |
Hyoscyamus niger | |
| 0.035 | - |
L-hyoscyamine | - |
Hyoscyamus niger | |
| 0.043 | - |
2-oxoglutarate | - |
Hyoscyamus niger | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | activates | Hyoscyamus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19000 | - |
x * 39000 + x * 19000, SDS-PAGE | Hyoscyamus niger |
| 39000 | - |
x * 39000 + x * 19000, SDS-PAGE | Hyoscyamus niger |
| 41000 | - |
gel filtration | Hyoscyamus niger |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-hyoscyamine + 2-oxoglutarate + O2 | Hyoscyamus niger | enzyme is involved in biosynthetic pathway leading to scopolamine | 6beta-hydroxyhyoscyamine + succinate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hyoscyamus niger | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hyoscyamus niger |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | culture | Hyoscyamus niger | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Hyoscyamus niger |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for more than 3 months | Hyoscyamus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-hydroxy-3-phenylpropionyltropine + 2-oxoglutarate + O2 | 15% of the activity with L-hyoscyamine | Hyoscyamus niger | 2-hydroxy-3-phenylpropionyl-6-hydroxytropine + succinate + CO2 | - |
? | |
| 2-oxoglutarate + p-hydroxyatropine + O2 | 26% of the activity with L-hyoscyamine | Hyoscyamus niger | ? | - |
? | |
| 2-oxoglutarate + trans-cinnamoyltropine + O2 | 39% of the activity with L-hyoscyamine | Hyoscyamus niger | succinate + trans-cinnamoyl-6-hydroxytropine + CO2 | - |
? | |
| 3-hydroxy-3-phenylpropionyltropine + 2-oxoglutarate + O2 | 56% of the activity with L-hyoscyamine | Hyoscyamus niger | 3-hydroxy-3-phenylpropionyl-6-hydroxytropine + succinate + CO2 | - |
? | |
| 6,7-dehydrohyoscyamine + 2-oxoglutarate + O2 | 119% of the activity with L-hyoscyamine | Hyoscyamus niger | scopolamine + succinate + CO2 | - |
? | |
| apoatropine + 2-oxoglutarate + O2 | 45% of the activity with L-hyoscyamine | Hyoscyamus niger | 6-hydroxyapoatropine + succinate + CO2 | - |
? | |
| isobutyltropine + 2-oxoglutarate + O2 | 15% of the activity with L-hyoscyamine | Hyoscyamus niger | ? | - |
? | |
| L-homatropine + 2-oxoglutarate + O2 | 81% of the activity with L-hyoscyamine | Hyoscyamus niger | 6-hydroxyhomatropine + succinate + CO2 | - |
? | |
| L-hyoscyamine + 2-oxoglutarate + O2 | - |
Hyoscyamus niger | 6beta-hydroxyhyoscyamine + succinate + CO2 | - |
? | |
| L-hyoscyamine + 2-oxoglutarate + O2 | enzyme is involved in biosynthetic pathway leading to scopolamine | Hyoscyamus niger | 6beta-hydroxyhyoscyamine + succinate + CO2 | - |
? | |
| L-norhyoscyamine + 2-oxoglutarate + O2 | 81% of the activity with L-hyoscyamine | Hyoscyamus niger | 6-hydroxynorhyoscyamine + succinate + CO2 | - |
? | |
| additional information | high specificity for 2-oxoglutarate in hydroxylation | Hyoscyamus niger | ? | - |
? | |
| noratropine-N-acetic acid + 2-oxoglutarate + O2 | 17% of the activity with L-hyoscyamine | Hyoscyamus niger | ? | - |
? | |
| phenylacetyltropine + 2-oxoglutarate + O2 | 81% of the activity with L-hyoscyamine | Hyoscyamus niger | 6-hydroxyphenylacetyltropine + succinate + CO2 | - |
? | |
| phenylalanyltropine + 2-oxoglutarate + O2 | 8% of the activity with L-hyoscyamine | Hyoscyamus niger | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 39000 + x * 19000, SDS-PAGE | Hyoscyamus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Hyoscyamus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | about 50% of maximal activity at pH 6.0 and pH 9.0 | Hyoscyamus niger |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.009 | - |
Pyridine 2,4-dicarboxylate | - |
Hyoscyamus niger | |
| 0.09 | - |
Pyridine 3,4-dicarboxylate | - |
Hyoscyamus niger | |
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