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Literature summary for 1.14.11.1 extracted from
- Cofactor requirements of gamma-butyrobetaine hydroxylase from rat liver (1970), J. Biol. Chem., 245, 4178-4186.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-amino-6,7-dimethyl-4-hydroxy-5,6,7,8-tetrahydropteridine | - |
Rattus norvegicus |  |
| catalase | - |
Rattus norvegicus | |
| isoascorbate | increase of activity | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-trimethylaminopropyl-1-sulfonate | - |
Rattus norvegicus | |
| arsenite | - |
Rattus norvegicus | |
| iodoacetate | less effective than p-chloromercuriphenylsulfonate | Rattus norvegicus | |
| Iodosobenzoate | less effective than p-chloromercuriphenylsulfonate | Rattus norvegicus | |
| N-ethylmaleimide | less effective than p-chloromercuriphenylsulfonate | Rattus norvegicus | |
| p-chloromercuribenzoate | inactivates enzyme completely at 0.1 mM | Rattus norvegicus | |
| p-chloromercuriphenylsulfonate | inactivates enzyme completely at 0.1 mM | Rattus norvegicus | |
| structure analogues of gamma-butyrobetaine and 2-oxoglutarate | - |
Rattus norvegicus | |
| Succinic semialdehyde | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Rattus norvegicus | |
| 0.5 | - |
2-oxoglutarate | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Rattus norvegicus | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | requirement | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.003 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-trimethylammoniobutanoate + 2-oxoglutarate + O2 | i.e. gamma-butyrobetaine | Rattus norvegicus | 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2 | i.e. L-carnitine | ? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | - |
- |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | half-maximal activity at pH 6.0 and 7.5 | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2,6-dichlorophenolindophenol | - |
Rattus norvegicus | |
| ascorbate | activation, keeps Fe2+ in the reduced state | Rattus norvegicus | |
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