Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.99.3 extracted from

  • Roberts, J.; Rosenfeld, H.J.
    Isolation, crystallization, and properties of indolyl-3-alkane alpha-hydroxylase. A novel tryptophan-metabolizing enzyme (1977), J. Biol. Chem., 252, 2640-2647.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
hydroxylamine
-
Pseudomonas sp.
KCN
-
Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe contains 0.8 mol of iron per mol of enzyme Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
250000
-
sedimentation equilibrium ultracentrifugation Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. XA
-
-
-

Reaction

Reaction Comment Organism Reaction ID
L-tryptophan + O2 = (indol-3-yl)glycolaldehyde + CO2 + NH3 primary reaction is hydroxylation of the side chain carbon adjacent to the indole ring, decarboxylation (of tryptophan only) is secondary Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14
-
-
Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-indolepropionate + O2
-
Pseudomonas sp. ?
-
?
3-indolepropionate + O2
-
Pseudomonas sp. XA ?
-
?
5-hydroxy-L-tryptophan + O2
-
Pseudomonas sp. ?
-
?
5-methyl-DL-tryptophan + O2
-
Pseudomonas sp. ?
-
?
DL-5-fluorotryptophan + O2
-
Pseudomonas sp. ?
-
?
indole-3-butyric acid + O2
-
Pseudomonas sp. ?
-
?
L-Trp-L-Phe + O2 + O2
-
Pseudomonas sp. ?
-
?
L-Trp-L-Trp + O2
-
Pseudomonas sp. ?
-
?
L-tryptophan + O2
-
Pseudomonas sp. 3-indoleglycolaldehyde + CO2 + NH3
-
?
L-tryptophan + O2
-
Pseudomonas sp. XA 3-indoleglycolaldehyde + CO2 + NH3
-
?
L-tryptophan methyl ester + O2
-
Pseudomonas sp. ?
-
?
additional information overview: specificity Pseudomonas sp. additional information
-
?
additional information substrate: tryptophan containing di- and oligopeptides Pseudomonas sp. additional information
-
?
additional information overview: specificity Pseudomonas sp. XA additional information
-
?
additional information substrate: tryptophan containing di- and oligopeptides Pseudomonas sp. XA additional information
-
?
N-acetyl-L-tryptophanamide + O2
-
Pseudomonas sp. N-acetyl-alpha,beta-didehydrotryptophanamide + H2O
-
?
N-acetyl-L-tryptophanamide + O2
-
Pseudomonas sp. XA N-acetyl-alpha,beta-didehydrotryptophanamide + H2O
-
?

Cofactor

Cofactor Comment Organism Structure
heme hemoprotein Pseudomonas sp.
heme contains 1.1 mol of heme per mol of enzyme Pseudomonas sp.