Literature summary for 1.13.99.1 extracted from

Chen, C.; Sun, X.; Duanmu, H.; Yu, Y.; Liu, A.; Xiao, J.; Zhu, Y.
Ectopic expression of a Glycine soja myo-inositol oxygenase gene (GsMIOX1a) in Arabidopsis enhances tolerance to alkaline stress (2015), PLoS ONE, 10, e0129998 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene AtMIOX1, phylogenetic tree, semi-quantitative reverse transcriptase enzyme expression analysis	Arabidopsis thaliana
gene GsMIOX1a, genetic structure, phylogenetic tree, ectopic expression under control of the constitutive CaMV35S promoter in Arabidopsis thaliana leads to enhanced tolerance to alkaline stress, while expression of enzyme mutant atmiox1 mutant decreases alkaline stress tolerance in Arabidopsis thaliana ecotype Columbia, quantitative real-time PCR enzyme expression analysis. The expression levels of some alkaline stress-responsive and inducible marker genes, including H+-Ppase, NADP-ME, KIN1 and RD29B, are also up-regulated in GsMIOX1a overexpression lines compared with the wild-type and atmiox1 mutant expressing plants	Glycine soja

Cloned (Comment)

Organism

gene AtMIOX1, phylogenetic tree, semi-quantitative reverse transcriptase enzyme expression analysis

Arabidopsis thaliana

gene GsMIOX1a, genetic structure, phylogenetic tree, ectopic expression under control of the constitutive CaMV35S promoter in Arabidopsis thaliana leads to enhanced tolerance to alkaline stress, while expression of enzyme mutant atmiox1 mutant decreases alkaline stress tolerance in Arabidopsis thaliana ecotype Columbia, quantitative real-time PCR enzyme expression analysis. The expression levels of some alkaline stress-responsive and inducible marker genes, including H+-Ppase, NADP-ME, KIN1 and RD29B, are also up-regulated in GsMIOX1a overexpression lines compared with the wild-type and atmiox1 mutant expressing plants

Glycine soja

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of an AtMIOX1 gene T-DNA insertion mutant atmiox1, the AtMIOX1 gene is efficiently silenced by the T-DNA insertion in the atmiox1 mutant. Constitutive overepression of Glycine soja MIOX1 under constitutive CaMV35S promoter control can recover the phenotype leading to alkaline stress tolerance in the atmiox1 mutant plants, overview	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
myo-inositol + O2	Arabidopsis thaliana	-	D-glucuronate + H2O	-	?
myo-inositol + O2	Glycine soja	-	D-glucuronate + H2O	-	?
myo-inositol + O2	Glycine soja 07256	-	D-glucuronate + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q8L799	-	-
Glycine soja	A0A0B2PCG9	-	-
Glycine soja 07256	A0A0B2PCG9	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
flower	GsMIOX1a expressed predominantly in flowers	Glycine soja	-
additional information	GsMIOX1a expression is tissue-specific	Glycine soja	-
seedling	-	Arabidopsis thaliana	-
seedling	-	Glycine soja	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
myo-inositol + O2	-	Arabidopsis thaliana	D-glucuronate + H2O	-	?
myo-inositol + O2	-	Glycine soja	D-glucuronate + H2O	-	?
myo-inositol + O2	-	Glycine soja 07256	D-glucuronate + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 36574, sequence calculation	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
AtMIOX	-	Arabidopsis thaliana
GsMIOX1a	-	Glycine soja
inositol oxygenase 1	-	Arabidopsis thaliana
inositol oxygenase 1	-	Glycine soja
MIOX	-	Glycine soja
Myo-inositol oxygenase	-	Glycine soja

pI Value

Organism	Comment	pI Value Maximum	pI Value
Arabidopsis thaliana	sequence calculation	-	4.93

Expression

Organism	Comment	Expression
Glycine soja	GsMIOX1a is rapidly induced by alkaline stress. Under alkaline stress, GsMIOX1a expression levels increase and reach a maximum level at 6 h that is approximately 42fold higher than that at 0 h	up

General Information

General Information	Comment	Organism
evolution	MIOX proteins are highly conserved and present in nearly all eukaryotes	Glycine soja
malfunction	the T-DNA insertion mutant atmiox1 is sensitive to alkaline stress, the phenotype can be complemented by expression of Glycine soja MIOX1 under constitutive CaMV35S promoter control	Arabidopsis thaliana
physiological function	positive function of enzyme GsMIOX1a in the alkaline response in Glycine soja plants, GsMIOX1a gene positively regulates plant tolerance to alkaline stress. Other soybean varieties, including Glycine soja 07256, Glycine soja 50109, Glycine max Suinong 28 and Glycine max Hefeng 55. Glycine max Suinong 28 and Glycine max Hefeng 55 are Chinese soybean cultivars that exhibit much lower adaptability to stress compared to Glycine soja 07256. Glycine soja 50109 is also a kind of wild soybean but is not reported to be alkaline resistant	Glycine soja