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Literature summary for 1.13.99.1 extracted from

  • Siddique, S.; Endres, S.; Sobczak, M.; Radakovic, Z.; Fragner, L.; Grundler, F.; Weckwerth, W.; Tenhaken, R.; Bohlmann, H.
    Myo-inositol oxygenase is important for the removal of excess myo-inositol from syncytia induced by Heterodera schachtii in Arabidopsis roots (2014), New Phytol., 201, 476-485 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
in Arabidpsis thaliana, myo-inositol oxygenase is encoded by four genes, gene MIOX1, quantitative real-time PCR enzyme expression analysis Arabidopsis thaliana
in Arabidpsis thaliana, myo-inositol oxygenase is encoded by four genes, gene MIOX2, quantitative real-time PCR enzyme expression analysis Arabidopsis thaliana
in Arabidpsis thaliana, myo-inositol oxygenase is encoded by four genes, gene MIOX4, quantitative real-time PCR enzyme expression analysis Arabidopsis thaliana
in Arabidpsis thaliana, myo-inositol oxygenase is encoded by four genes, gene MIOX5, quantitative real-time PCR enzyme expression analysis Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of a quadruple (miox1/2/4/5) mutant by mutation of all four MIOX isozymes via T-DNA insertion, metabolite profiling of syncytia induced in roots of the myo-inositol oxygenase quadruple mutant, overview. The quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility can also be achieved by exogenous application of galactinol to wild-type seedlings. The miox1/2/4/5 plants show a similar reduction in the number of females to miox double mutants Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol + O2 Arabidopsis thaliana
-
D-glucuronate + H2O
-
?
myo-inositol + O2 Arabidopsis thaliana Col-0
-
D-glucuronate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O82200
-
-
Arabidopsis thaliana Q8H1S0
-
-
Arabidopsis thaliana Q8L799
-
-
Arabidopsis thaliana Q9FJU4
-
-
Arabidopsis thaliana Col-0 O82200
-
-
Arabidopsis thaliana Col-0 Q8H1S0
-
-
Arabidopsis thaliana Col-0 Q8L799
-
-
Arabidopsis thaliana Col-0 Q9FJU4
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pollen MIOX4 is highly expressed in pollen Arabidopsis thaliana
-
pollen MIOX5 is highly expressed in pollen Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-
seedling MIOX1 is expressed preferentially in seedlings Arabidopsis thaliana
-
seedling MIOX2 is expressed preferentially in seedlings Arabidopsis thaliana
-
syncytium high MIOX1 expression level in syncytia, induced in roots by attack through the beet cyst nematode Heterodera schachtii, metabolome analysis of syncytium, overview Arabidopsis thaliana
-
syncytium high MIOX2 expression level in syncytia, induced in roots by attack through the beet cyst nematode Heterodera schachtii, metabolome analysis of syncytium, overview Arabidopsis thaliana
-
syncytium high MIOX4 expression level in syncytia, induced in roots by attack through the beet cyst nematode Heterodera schachtii, metabolome analysis of syncytium, overview Arabidopsis thaliana
-
syncytium high MIOX5 expression level in syncytia, induced in roots by attack through the beet cyst nematode Heterodera schachtii, metabolome analysis of syncytium, overview Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol + O2
-
Arabidopsis thaliana D-glucuronate + H2O
-
?
myo-inositol + O2
-
Arabidopsis thaliana Col-0 D-glucuronate + H2O
-
?

Synonyms

Synonyms Comment Organism
MIOX
-
Arabidopsis thaliana
MIOX1
-
Arabidopsis thaliana
MIOX2
-
Arabidopsis thaliana
MIOX4
-
Arabidopsis thaliana
MIOX5
-
Arabidopsis thaliana
Myo-inositol oxygenase
-
Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana all MIOX genes are strongly expressed in syncytia induced by the beet cyst nematode Heterodera schachtii in Arabidopsis thaliana roots up

General Information

General Information Comment Organism
malfunction the quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility can also be achieved by exogenous application of galactinol to wild-type seedlings. Except for MIOX2, transcripts for the other three MIOX genes are still detected at a level of 2-14% of their abundance in the wild-type, the mutant does not show any visible phenotype and produced viable pollen, but the incorporation of myo-inositol-derived sugars into cell walls is strongly inhibited by over 90% with no change in the ultrastructure of syncytial cell walls Arabidopsis thaliana
physiological function the enzyme myo-inositol oxygenase is the key enzyme of a pathway leading from myo-inositol to UDP-glucuronic acid. The primary function of myo-inositol oxygenase for syncytium development after infection with Heterodera schachtii is probably not the production of UDP-glucuronic acid as a precursor for cell wall polysaccharides, but the reduction of myo-inositol levels and thereby a reduction in the galactinol level to avoid the induction of defence-related genes Arabidopsis thaliana