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Literature summary for 1.13.12.9 extracted from

  • Suzuki, H.; Higashi, Y.; Asano, M.; Suguro, M.; Kigawa, M.; Maeda, M.; Katayama, S.; Mukouyama, E.B.; Uchiyama, K.
    Sequencing and expression of the L-phenylalanine oxidase gene from Pseudomonas sp. P-501. Proteolytic activation of the proenzyme (2004), J. Biochem., 136, 617-627.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) as His-tag fusion protein Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
L-phenylalanine pH 8.0, 25°C Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-phenylalanine + O2 Pseudomonas sp.
-
2-phenylacetamide + CO2 + H2O
-
?
L-phenylalanine + O2 Pseudomonas sp. P-501
-
2-phenylacetamide + CO2 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. Q5W9R9
-
-
Pseudomonas sp. P-501 Q5W9R9
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme using His-tag Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + O2
-
Pseudomonas sp. 2-phenylacetamide + CO2 + H2O
-
?
L-phenylalanine + O2
-
Pseudomonas sp. P-501 2-phenylacetamide + CO2 + H2O
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
103
-
L-phenylalanine pH 8.0, 25°C Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD noncovalently bound Pseudomonas sp.