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Literature summary for 1.13.12.8 extracted from

  • Gimenez, G.; Metcalf, P.; Paterson, N.G.; Sharpe, M.L.
    Mass spectrometry analysis and transcriptome sequencing reveal glowing squid crystal proteins are in the same superfamily as firefly luciferase (2016), Sci. Rep., 6, 27638 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.12.8

Cloned(Commentary)

Cloned (Comment) Organism
three proteins termed wsluc1 (encoded by transcript 82699_c0_seq1), wsluc2 (81000_c2_seq2) and wsluc3 (83251_c0_seq1), sequence comparisons and phylogenetic analysis, transcriptome analysis Watasenia scintillans

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information insoluble Watasenia scintillans
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Watasenia luciferin + O2 Watasenia scintillans coelenterazine disulfate is the Watasenia scintillans luciferin substrate, and the reaction requires ATP, Mg2+ and molecular oxygen. Protein microcrystals in the arm photophores catalyse the bioluminescent reaction using ATP and the substrate coelenterazine disulfate oxidized Watasenia luciferin + CO2 + hnu
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Organism

Organism UniProt Comment Textmining
Watasenia scintillans
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Source Tissue

Source Tissue Comment Organism Textmining
photophore photophores at the tips of two arms, the photophores are densely packed with protein microcrystals that catalyse the bioluminescent reaction using ATP and the substrate coelenterazine disulfate Watasenia scintillans
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Watasenia luciferin + O2
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 Watasenia scintillans oxidized Watasenia luciferin + CO2 + hnu
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Watasenia luciferin + O2 coelenterazine disulfate is the Watasenia scintillans luciferin substrate, and the reaction requires ATP, Mg2+ and molecular oxygen. Protein microcrystals in the arm photophores catalyse the bioluminescent reaction using ATP and the substrate coelenterazine disulfate Watasenia scintillans oxidized Watasenia luciferin + CO2 + hnu
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 ?

Subunits

Subunits Comment Organism
More protein crystals show two bands of approximately 59 and 81 kDa in a ratio of 4:1 or 5:1 Watasenia scintillans

General Information

General Information Comment Organism
evolution proteins wsluc1-3 belong to the ANL superfamily. ANL superfamily enzymes have independently evolved in distant species to produce light using unrelated substrates Watasenia scintillans
additional information protein microcrystals in the arm photophores catalyse the bioluminescent reaction using ATP and the substrate coelenterazine disulfate. The crystals contain a major 63 kDa protein and a minor 81 kDa protein, in a mass ratio of about 8 to 1. Three homologous proteins comprise the luminescent arm tip microcrystals, i.e. proteins wsluc1-3, wsluc1-3 form a complex that crystallises inside the squid photophores. Analysis of the proteins from the protein crystal extraction identified by MALDI TOF/TOF mass spectrometry analysis, overview Watasenia scintillans