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Literature summary for 1.13.12.7 extracted from
- Magnetic nanoparticles supported ionic liquids improve firefly luciferase properties (2014), Appl. Biochem. Biotechnol., 172, 3116-3127 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | enzyme activity and structural stability increases in presence of magnetic magnetite (lambda-Fe2O3) nanoparticles supported ionic liquids. The effect of ingredients which are used is not considerable on Km value of luciferase for ATP and also Km value for luciferin, synthesis and evaluation, overview. The use of ionic liquids supported on magnetic nanoparticles improves kinetic and structural properties of firefly luciferase using bioluminescence assay, fluorescence spectroscopy, and circular dichroism | Photinus pyralis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | irreversible aggregation because of the exposure of its hydrophobic sites followed by structural changes leads to its further inactivation | Photinus pyralis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-firefly luciferin + O2 + ATP | Photinus pyralis | - |
firefly oxyluciferin + CO2 + AMP + diphosphate + hv | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Photinus pyralis | P08659 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-firefly luciferin + O2 + ATP | - |
Photinus pyralis | firefly oxyluciferin + CO2 + AMP + diphosphate + hv | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| firefly luciferase | - |
Photinus pyralis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
free wild-type enzyme | Photinus pyralis |
| 30 | - |
enzyme in presence of magnetic magnetite nanoparticles supported ionic liquids | Photinus pyralis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermal stability and activity at 35°C of the enzyme in presence of magnetic magnetite nanoparticles supported ionic liquids is increased compared to the free enzyme, overview | Photinus pyralis |
| 35 | 45 | free enzyme shows 65% remaning activity after 10 min at 35°C, inactivation after 4 min at 35°C, inactivation at 45°C | Photinus pyralis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Photinus pyralis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Photinus pyralis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | firefly luciferase from North American Photinus pyralis is a monooxygenase that performs ATP-dependent conversion of luciferin into a luciferyl-adenylate, which is oxidized in a multistep reaction to electronically excited oxyluciferin | Photinus pyralis |
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Release 2023.1 (January 2023)
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