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Literature summary for 1.13.12.7 extracted from
- Implication of Arg213 and Arg337 on the kinetic and structural stability of firefly luciferase (2013), Int. J. Biol. Macromol., 52, 157-163.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed in Escherichia coli BL-21 | Photinus pyralis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R213M | Cm (M): 1.8 (wild-type: 2.4), T1/2 (min): 19.5 (wild-type: 19.5), Tm (°C): 46.5 (wild-type: 46.5), activation energy (kcal/mol): 2.59 (wild-type: 6.51), [urea]50% (M): 2.86 (wild-type: 1.35) | Photinus pyralis |
| R337Q | Cm (M): 1.7 (wild-type: 2.4), T1/2 (min): 22.1 (wild-type: 19.5), Tm (°C): 50.7 (wild-type: 46.5), activation energy (kcal/mol): 3.39 (wild-type: 6.51), [urea]50% (M): 2.18 (wild-type: 1.35) | Photinus pyralis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
SDS-PAGE | Photinus pyralis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Photinus pyralis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Photinus pyralis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-luciferin + ATP + O2 | - |
Photinus pyralis | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| luciferin | - |
Photinus pyralis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Photinus pyralis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
stability remains constant upon 120 min | Photinus pyralis |
| 30 | - |
T1/2 (min): 16.2 (wild-type), 19.5 (mutant R213M), 22.1 (mutant R337Q) | Photinus pyralis |
| 37 | - |
high loss of activity in less than 10 min | Photinus pyralis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Photinus pyralis |
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Release 2023.1 (January 2023)
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