Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha-synuclein | dose-dependent enhancement of oxyluciferin formation, maximum rate of bioluminescence independent upon ATP concentration | Photinus pyralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.074 | - |
Luciferin | 0.5 mM ATP, in presence of alpha-synuclein | Photinus pyralis | |
0.086 | - |
ATP | 0.094 mM luciferin, in presence of alpha-synuclein | Photinus pyralis | |
0.088 | - |
Luciferin | 0.2 mM ATP, in presence of alpha-synuclein | Photinus pyralis | |
0.114 | - |
ATP | 0.094 mM luciferin, without alpha-synuclein | Photinus pyralis | |
0.114 | - |
ATP | 0.188 mM luciferin, in presence of alpha-synuclein | Photinus pyralis | |
0.134 | - |
Luciferin | 0.15 mM ATP, in presence of alpha-synuclein | Photinus pyralis | |
0.157 | - |
ATP | 0.188 mM luciferin, without alpha-synuclein | Photinus pyralis | |
0.188 | - |
Luciferin | 0.15 mM ATP, without alpha-synuclein | Photinus pyralis | |
0.189 | - |
Luciferin | 0.5 mM ATP, without alpha-synuclein | Photinus pyralis | |
0.192 | - |
Luciferin | 0.2 mM ATP, without alpha-synuclein | Photinus pyralis | |
0.197 | - |
Luciferin | 0.1 mM ATP, without alpha-synuclein | Photinus pyralis | |
0.218 | - |
Luciferin | 0.1 mM ATP, in presence of alpha-synuclein | Photinus pyralis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Photinus pyralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photinus pyralis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
luciferin + ATP + O2 | - |
Photinus pyralis | oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? |
Synonyms | Comment | Organism |
---|---|---|
firefly luciferase | - |
Photinus pyralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Photinus pyralis |