Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.12.7 extracted from

  • Tafreshi, N.K.h.; Sadeghizadeh, M.; Emamzadeh, R.; Ranjbar, B.; Naderi-Manesh, H.; Hosseinkhani, S.
    Site-directed mutagenesis of firefly luciferase: implication of conserved residue(s) in bioluminescence emission spectra among firefly luciferases (2008), Biochem. J., 412, 27-33.
    View publication on PubMed

Application

Application Comment Organism
biotechnology imaging technology Lampyris turkestanicus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Lampyris turkestanicus

Protein Variants

Protein Variants Comment Organism
H245N red- and green-emitting luciferase, increase in thermostability, about 25% decrease in relative activity Lampyris turkestanicus
H431Y red- and green-emitting luciferase, about 85% decrease in relative acitivity Lampyris turkestanicus
S284T red-emitting luciferase, about 75% decrease in relative activity Lampyris turkestanicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01612
-
D-luciferin wild-type luciferase Lampyris turkestanicus
0.023
-
D-luciferin mutant H245N Lampyris turkestanicus
0.026
-
D-luciferin mutant H431Y Lampyris turkestanicus
0.03
-
D-luciferin mutant S284T Lampyris turkestanicus
0.135
-
ATP wild-type luciferase Lampyris turkestanicus
0.168
-
ATP mutant H245N Lampyris turkestanicus
0.1872
-
ATP mutant H431Y Lampyris turkestanicus
0.2484
-
ATP mutant S284T Lampyris turkestanicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Lampyris turkestanicus

Organism

Organism UniProt Comment Textmining
Lampyris turkestanicus Q5UFR2 wild-type emits green light
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA Sepharose Lampyris turkestanicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
11500000000000 relative light units/mg, mutant H245N Lampyris turkestanicus
additional information
-
15000000000000 relative light units/mg, wild-type luciferase Lampyris turkestanicus
additional information
-
2500000000000 relative light units/mg, mutant H431Y Lampyris turkestanicus
additional information
-
3600000000000 relative light units/mg, mutant S284T Lampyris turkestanicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-luciferin + ATP + O2 assay at 25°C, pH 7.8 Lampyris turkestanicus oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
?

Synonyms

Synonyms Comment Organism
firefly luciferase
-
Lampyris turkestanicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
24
-
wild-type luciferase, mutant H245N and H431Y Lampyris turkestanicus
25
-
assay at Lampyris turkestanicus
30
-
mutant S284T Lampyris turkestanicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
15 40 at 35°C native luciferase completely inactive, mutant H245N still 80% residual activity, mutant S284T and H431Y about 20% residual activity Lampyris turkestanicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Lampyris turkestanicus
8
-
wild-type luciferase, mutant S284T Lampyris turkestanicus
8.5
-
mutant H245N and H431Y Lampyris turkestanicus