Literature summary for 1.13.12.7 extracted from

Svetlov, M.S.; Kolb, V.A.; Spirin, A.S.
Folding of the firefly luciferase polypeptide chain with the immobilized C terminus (2007), Mol. Biol., 41, 86-92.

No PubMed abstract available

Search for more literature for 1.13.12.7

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) cells	Photinus pyralis

Organism

Organism	UniProt	Comment	Textmining
Photinus pyralis	Q27758	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-Sepharose column chromatography	Photinus pyralis

Renatured (Commentary)

Renatured (Comment)	Organism
renaturation is initiated by diluting the denaturant in 50 mM HEPES-KOH, pH 7.5, 10 mM Mg(OAc)2, 70 mM KOAc, 50 mM imidazole, and 1 mM dithiothreitol without urea, refolding of firefly luciferase from a denatured state is a slow process, its rate and productivity depend on molecular chaperones of the Hsp70 family, Hsp70-dependent refolding restores 55% of the initial enzymatic activity, immobilization leads to a higher refolding yield owing to the prevention of intermolecular aggregation	Photinus pyralis

Renatured (Comment)

Organism

renaturation is initiated by diluting the denaturant in 50 mM HEPES-KOH, pH 7.5, 10 mM Mg(OAc)2, 70 mM KOAc, 50 mM imidazole, and 1 mM dithiothreitol without urea, refolding of firefly luciferase from a denatured state is a slow process, its rate and productivity depend on molecular chaperones of the Hsp70 family, Hsp70-dependent refolding restores 55% of the initial enzymatic activity, immobilization leads to a higher refolding yield owing to the prevention of intermolecular aggregation

Photinus pyralis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-luciferin + O2 + ATP	-	Photinus pyralis	oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv	-	?

Synonyms

Synonyms	Comment	Organism
firefly luciferase	-	Photinus pyralis
Luc	-	Photinus pyralis

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Release 2023.1 (January 2023)