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Literature summary for 1.13.12.7 extracted from

  • Shapiro, E.; Lu, C.; Baneyx, F.
    A set of multicolored Photinus pyralis luciferase mutants for in vivo bioluminescence applications (2005), Protein Eng. Des. Sel., 18, 581-587.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Photinus pyralis

Protein Variants

Protein Variants Comment Organism
L287I orange light emitting mutant, mutation does not affect the structural integrity and/or folding of luciferase Photinus pyralis
Q283R red light emitting mutant, mutation does not affect the structural integrity and/or folding of luciferase Photinus pyralis
S284G red light emitting mutant, mutation does not affect the structural integrity and/or folding of luciferase Photinus pyralis
S293P orange light emitting mutant, mutation does not affect the structural integrity and/or folding of luciferase Photinus pyralis

Organism

Organism UniProt Comment Textmining
Photinus pyralis
-
-
-

Synonyms

Synonyms Comment Organism
Luc
-
Photinus pyralis
Photinus pyralis luciferase
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Photinus pyralis

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
all spectra undergo a red-shift when cells are assayed under acidic conditions, whereas a blue-shift is observed at pH 8.0 Photinus pyralis