Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzymes are expressed as GST-fusion proteins | Photinus pyralis |
wild-type and mutant enzymes are expressed as GST-fusion proteins | Pyrophorus plagiophthalamus |
Protein Variants | Comment | Organism |
---|---|---|
A243G | bioluminescence emission maximum with luciferyl-O-adenosine monophosphate is 599 nm compared to 549 nm for the wild-type enzyme, bioluminescence emission maxima with 5,5-dimethyl-luciferyl-O-adenosine monophosphate are 610 nm and 557 nm compared to 624 nm for the wild-type enzyme | Pyrophorus plagiophthalamus |
F250S | bioluminescence emission maximum with luciferyl-O-adenosine monophosphate is 546 nm compared to 552 nm for the wild-type enzyme, bioluminescence emission maxima with 5,5-dimethyl-luciferyl-O-adenosine monophosphate are 631 nm and 552 nm compared to 560 nm for the wild-type enzyme | Photinus pyralis |
G246A | bioluminescence emission maximum with luciferyl-O-adenosine monophosphate is 548 nm compared to 552 nm for the wild-type enzyme, bioluminescence emission maximum with 5,5-dimethyl-luciferyl-O-adenosine monophosphate is 578 nm compared to 560 nm for the wild-type enzyme | Photinus pyralis |
S247F | bioluminescence emission maximum with luciferyl-O-adenosine monophosphate is 597 nm compared to 549 nm for the wild-type enzyme, bioluminescence emission maximum with 5,5-dimethyl-luciferyl-O-adenosine monophosphate is 612 nm compared to 624 nm for the wild-type enzyme | Pyrophorus plagiophthalamus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
5,5-dimethyl-luciferyl-O-adenosine monophosphate | pH 8.6, wild-type enzyme | Photinus pyralis | |
0.0003 | - |
5,5-dimethyl-luciferyl-O-adenosine monophosphate | pH 8.6, mutant enzyme F250S | Photinus pyralis | |
0.0003 | - |
5,5-dimethyl-luciferyl-O-adenosine monophosphate | pH 8.6, mutant enzyme G246A | Photinus pyralis | |
0.0003 | - |
5,5-dimethyl-luciferyl-O-adenosine monophosphate | pH 8.6, wild-type enzyme | Pyrophorus plagiophthalamus | |
0.0004 | - |
5,5-dimethyl-luciferyl-O-adenosine monophosphate | pH 8.6, mutant enzyme A243G | Pyrophorus plagiophthalamus | |
0.0006 | - |
luciferyl-O-adenosine monophosphate | pH 8.6, mutant enzyme A243G | Pyrophorus plagiophthalamus | |
0.0008 | - |
5,5-dimethyl-luciferyl-O-adenosine monophosphate | pH 8.6, mutant enzyme S247F | Pyrophorus plagiophthalamus | |
0.0008 | - |
luciferyl-O-adenosine monophosphate | pH 8.6, wild-type enzyme | Pyrophorus plagiophthalamus | |
0.0046 | - |
luciferyl-O-adenosine monophosphate | pH 8.6, mutant enzyme S247F | Pyrophorus plagiophthalamus | |
0.0077 | - |
luciferyl-O-adenosine monophosphate | pH 8.6, wild-type enzyme | Photinus pyralis | |
0.0114 | - |
luciferyl-O-adenosine monophosphate | pH 8.6, mutant enzyme F250S | Photinus pyralis | |
0.0127 | - |
luciferyl-O-adenosine monophosphate | pH 8.6, mutant enzyme G246A | Photinus pyralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photinus pyralis | - |
- |
- |
Pyrophorus plagiophthalamus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Photinus pyralis |
Storage Stability | Organism |
---|---|
4°C, 20% loss of activity after 2 weeks | Pyrophorus plagiophthalamus |
4°C, wild-type and mutant enzymes remain fully active for up to 6 months | Photinus pyralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5-dimethyl-luciferyl-O-adenosine monophosphate + ? | - |
Photinus pyralis | 5,5-dimethyloxyluciferin + hv | - |
? | |
5,5-dimethyl-luciferyl-O-adenosine monophosphate + ? | - |
Pyrophorus plagiophthalamus | 5,5-dimethyloxyluciferin + hv | - |
? | |
luciferin + O2 + ATP | - |
Photinus pyralis | oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv | luciferase modulates emission color by controlling the resonance-based delocalization of the anionic keto form of the oxyluciferin excited state | ? | |
luciferin + O2 + ATP | - |
Pyrophorus plagiophthalamus | oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv | luciferase modulates emission color by controlling the resonance-based deloclization of the anionic keto form of the oxyluciferin excited state | ? | |
luciferyl-O-adenosine monophosphate + ? | - |
Photinus pyralis | ? | - |
? | |
luciferyl-O-adenosine monophosphate + ? | - |
Pyrophorus plagiophthalamus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
reaction with 5,5-dimethyl-luciferyl-O-adenosine monophosphate | Photinus pyralis |