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Literature summary for 1.13.12.7 extracted from

  • Lee, R.; McElroy, W.D.
    Role and reactivity of sulfhydryl groups in firefly luciferase (1969), Biochemistry, 8, 130-135.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide
-
Photinus pyralis
N-ethylmaleimide
-
Photinus pyralis
N-tosyl-L-lysine chloromethyl ketone
-
Photinus pyralis
N-tosyl-L-phenylalanine chloromethyl ketone competitive inhibitor with respect to luciferin, noncompetitive with respect to ATP, pH dependent, enzyme protected by dehydroluciferin-AMP Photinus pyralis
p-mercuribenzoate
-
Photinus pyralis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
Luciferin
-
Photinus pyralis

Organism

Organism UniProt Comment Textmining
Photinus pyralis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Photinus luciferin + O2 + ATP
-
Photinus pyralis oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.25
-
N-tosyl-L-phenylalanine chloromethyl ketone
-
Photinus pyralis
0.73
-
N-tosyl-L-lysine chloromethyl ketone
-
Photinus pyralis