Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Luciferin | preparations supplemented with extra luciferin provide maximum light emission | Photinus pyralis |
General Stability | Organism |
---|---|
bovine serum albumin stabilizes | Photinus pyralis |
dithiothreitol stabilizes | Photinus pyralis |
EDTA stabilizes | Photinus pyralis |
more stable in phosphate buffer than in Tricine buffer | Photinus pyralis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Photinus pyralis | |
EDTA | - |
Photinus pyralis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Photinus pyralis | |
Mg2+ | concentration 5 mM, MgSO4, MgCl2, Mg(O2CCH3)2 equally suitable salts | Photinus pyralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photinus pyralis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Photinus luciferin + O2 + ATP | the true substrate is MgATP2- | Photinus pyralis | oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Photinus pyralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Photinus pyralis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
buffers with pKa from 7.2 to 8.4 tested and no relationship with activity found | Photinus pyralis |
6.5 | 8.5 | pH 6.5: about 45% of activity maximum, pH 8.5: about 35% of activity maximum | Photinus pyralis |