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Literature summary for 1.13.12.7 extracted from

  • Rajgopal S; Vijayalakshmi M.A.
    Firefly luciferase: purification and immobilization (1984), Enzyme Microb. Technol., 6, 482-490.
No PubMed abstract available
Search for more literature for 1.13.12.7

Crystallization (Commentary)

Crystallization (Comment) Organism
extraction, 2 gel elutions, 5 crystallizations Photinus pyralis

General Stability

General Stability Organism
dithiothreitol stabilizes Photinus pyralis
stability increases 1000fold with immobilization on CNBr-Sepharose 4B, pH 7.5 at 25°C Luciola mingrelica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 from sedimentation velocity of the crystalline form Photinus pyralis

Organism

Organism UniProt Comment Textmining
Luciola mingrelica
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Photinus pyralis
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-
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Purification (Commentary)

Purification (Comment) Organism
chromatography on DEAE-cellulose Luciola mingrelica
several chromatographic and non chromatographic methods Photinus pyralis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Photinus pyralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Photinus luciferin + O2 + ATP
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 Photinus pyralis oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
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 ?

Subunits

Subunits Comment Organism
dimer 1 * alpha (catalytic subunit) + 1 * beta Photinus pyralis
dimer two non identical subunits Photinus pyralis