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Literature summary for 1.13.12.7 extracted from

  • Palomares J.P.; DeLuca M.A.Helinski D.R.
    Firefly luciferase enzyme for measuring gene expression in vegetative and symbiotic Rhizobium meliloti and other gram-negative bacteria (1989), Gene, 81, 155-64.
No PubMed abstract available
Search for more literature for 1.13.12.7

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Photinus pyralis
gene expression in vegetative and symbiotic Rhizobium melioti and other gram-negative bacteria Photinus pyralis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+, Ca2+, Zn2+, Ni2+, Cu2+, Fe3+, Li+, Na+, K+ not required for activity Photinus pyralis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
-
 Photinus pyralis

Organism

Organism UniProt Comment Textmining
Photinus pyralis
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 quantum yield 0.88 with respect to luciferin Photinus pyralis
additional information
-
 detection of activity in light units/mg of protein Photinus pyralis
additional information
-
 luminometer measures in Escherichia coli, Rhizobium melioti, Medicago sativa Photinus pyralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Photinus luciferin + O2 + ATP
-
 Photinus pyralis oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Photinus pyralis