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Literature summary for 1.13.12.6 extracted from

  • Morita, N.; Haga, S.; Ohmiya, Y.; Ozaki, M.
    Long-term ex vivo and in vivo monitoring of tumor progression by using dual luciferases (2016), Anal. Biochem., 497, 24-26 .
    View publication on PubMed

Application

Application Comment Organism
analysis the secreted Cypridina luciferase (CLuc) is used as an ex vivo indicator to continuously monitor tumor progression. On the other hand, the non-secreted firefly luciferase is used as an in vivo indicator to analyze the spatial distribution of the tumor at suitable time points indicated by CLuc. Tumor monitoring systems using dual luciferases are available, allowing long-term bioluminescence imaging under minimal stress for the experimental animals, e.g. BALB/cAJcl-nu/nu mice. Continuous non-invasive measurement of CLuc activity for tumor growth allows us to determine a suitable time to assess tumor spatial distribution and growth, thereby, reducing the animal stress and the experimental cost Cypridina noctiluca

Protein Variants

Protein Variants Comment Organism
additional information concept of tumor monitoring using dual luciferases, construction of the expression vector, and evaluation of tumor monitoring systems using dual luciferases from Cypridina noctiluca and firefly Photinus pyralis, overview. The enzymes are expressed in human breast cancercells MDA-MB-231, followed by inoculatin of the MDA-MB-231/FIC cell suspension subcutaneously into the back of 6-week-old male nude mice lacking T-cell function (BALB/cAJcl-nu/nu). The expressed CLuc is secreted into the blood from the cells and circulates in the living body. The blood containing CLuc can be drawn by using glass micro-hematocrit capillary tubes or a syringe Cypridina noctiluca

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Cypridina noctiluca
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Organism

Organism UniProt Comment Textmining
Cypridina noctiluca Q75R40
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Synonyms

Synonyms Comment Organism
CLuc
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Cypridina noctiluca
Cypridina luciferase
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Cypridina noctiluca