Application | Comment | Organism |
---|---|---|
analysis | the secreted Cypridina luciferase (CLuc) is used as an ex vivo indicator to continuously monitor tumor progression. On the other hand, the non-secreted firefly luciferase is used as an in vivo indicator to analyze the spatial distribution of the tumor at suitable time points indicated by CLuc. Tumor monitoring systems using dual luciferases are available, allowing long-term bioluminescence imaging under minimal stress for the experimental animals, e.g. BALB/cAJcl-nu/nu mice. Continuous non-invasive measurement of CLuc activity for tumor growth allows us to determine a suitable time to assess tumor spatial distribution and growth, thereby, reducing the animal stress and the experimental cost | Cypridina noctiluca |
Protein Variants | Comment | Organism |
---|---|---|
additional information | concept of tumor monitoring using dual luciferases, construction of the expression vector, and evaluation of tumor monitoring systems using dual luciferases from Cypridina noctiluca and firefly Photinus pyralis, overview. The enzymes are expressed in human breast cancercells MDA-MB-231, followed by inoculatin of the MDA-MB-231/FIC cell suspension subcutaneously into the back of 6-week-old male nude mice lacking T-cell function (BALB/cAJcl-nu/nu). The expressed CLuc is secreted into the blood from the cells and circulates in the living body. The blood containing CLuc can be drawn by using glass micro-hematocrit capillary tubes or a syringe | Cypridina noctiluca |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Cypridina noctiluca | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cypridina noctiluca | Q75R40 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
CLuc | - |
Cypridina noctiluca |
Cypridina luciferase | - |
Cypridina noctiluca |