Application | Comment | Organism |
---|---|---|
pharmacology | investigations into regulation and functional roles of kinases | Renilla sp. |
Cloned (Comment) | Organism |
---|---|
expressed in HEK-293T cells | Renilla sp. |
expression of RLuc8 in Escherichia coli BL21 | Renilla sp. |
Protein Variants | Comment | Organism |
---|---|---|
RLuc8 | eight-point mutation variant of renilla luciferase | Renilla sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H-89 | 10 microM, reduced luminescence to 50% of RLuc and to 54% of RLuc8 | Renilla sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Renilla sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Renilla sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
b-coelenterazine + O2 | - |
Renilla sp. | ? | - |
? | |
D-luciferin + O2 | - |
Renilla sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
firefly luciferase | - |
Renilla sp. |
fluc | - |
Renilla sp. |
Renilla luciferase | - |
Renilla sp. |
RLuc | - |
Renilla sp. |
RLuc8 | eight-point mutation variant of RLuc | Renilla sp. |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.021 | - |
10 microM coelenterazine, measured RLuc8 activity | Renilla sp. | H-89 |