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Literature summary for 1.13.12.5 extracted from

  • Kaihara, A.; Umezawa, Y.; Furukawa, T.
    Bioluminescent indicators for Ca2+ based on split renilla luciferase complementation in living cells (2008), Anal. Sci., 24, 1405-1408.
    View publication on PubMed
Search for more literature for 1.13.12.5

Activating Compound

Activating Compound Comment Organism Structure
ATP 50 microM Renilla sp.
histamine 10 microM Renilla sp.
additional information Ca2+-induced interaction between calmodulin and M13 leads to intermolecular complementation of split Renilla luciferase Renilla sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain DH5alpha and MCF-7 cells Renilla sp.

Protein Variants

Protein Variants Comment Organism
C124A increased enzymatic activity, 41fold higher luminescence than wild-type luciferase Renilla sp.
C124Ac C-terminal half, decreased enzymatic activity Renilla sp.
C124An N-terminal half, decreased enzymatic activity Renilla sp.
N64C Ca2+-induced interaction between calmodulin and M13 leads to intermolecular complementation of split Renilla luciferase, decreased enzymatic activity Renilla sp.

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+-induced interaction between calmodulin and M13 leads to intermolecular complementation of split Renilla luciferase Renilla sp.

Organism

Organism UniProt Comment Textmining
Renilla sp.
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 11900000000 luminescence intensity per microg protein Renilla sp.
additional information
-
 16300000000 luminescence intensity per microg protein, mutant N64C Renilla sp.
additional information
-
 3860000 luminescence intensity per microg protein, mutant C124Ac Renilla sp.
additional information
-
 481000000000 luminescence intensity per microg protein, mutant C124A Renilla sp.
additional information
-
 67400000 luminescence intensity per microg protein, mutant C124An Renilla sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coelenterazine + O2
-
 Renilla sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
Renilla luciferase
-
 Renilla sp.