Activating Compound | Comment | Organism | Structure |
---|---|---|---|
coelenterazine | increase of wild-type Gluc activity at higher substrate concentrations | Gaussia princeps | |
Triton X-100 | 0.1%, 3.7fold increase of specific activity of wild-tpye Gluc | Gaussia princeps |
Application | Comment | Organism |
---|---|---|
molecular biology | sensitive reproter for studies of gene expression, promoter activity, protein-protein interactions, signal transduction, tumor cell growth, response to therapy | Gaussia princeps |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli DH10B cells and 293T human kidney fibroblasts | Gaussia princeps |
Protein Variants | Comment | Organism |
---|---|---|
M43I | after 120 s of incubation time still 87% residual light emission compared to only 30% of wild-type Gluc, stabilized light emission with detergent Triton X-100, more stable light emission compared to wild-type Gluc when expression in mammalian cells | Gaussia princeps |
additional information | without the signal sequence higher enzyme yield and activity | Gaussia princeps |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | secreted | Gaussia princeps | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
SDS-PAGE | Gaussia princeps |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gaussia princeps | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gaussia princeps |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
value about 100000000000 relative light units/mg protein, wild-type Gluc, in presence of Triton X-100 | Gaussia princeps |
additional information | - |
value about 20000000000 relative light units/mg protein, mutant M43I | Gaussia princeps |
additional information | - |
value about 20000000000 relative light units/mg protein, wild-type Gluc | Gaussia princeps |
additional information | - |
value about 30000000000 relative light units/mg protein, mutant M43I, in presence of Triton X-100 | Gaussia princeps |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coelenterazine + O2 | 20 microM, assay at pH 8.0 | Gaussia princeps | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Gaussia luciferase | - |
Gaussia princeps |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Gaussia princeps |