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Literature summary for 1.13.12.5 extracted from

  • Loening, A.M.; Fenn, T.D.; Wu, A.M.; Gambhir, S.S.
    Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output (2006), Protein Eng. Des. Sel., 19, 391-400.
    View publication on PubMed
Search for more literature for 1.13.12.5

Application

Application Comment Organism
molecular biology the development of variants of Renilla luciferase, which exhibit significantly improved properties compared with the native enzyme, will allow enhanced sensitivity in existing luciferase-based assays as well as enable the development of novel probes labeled with the luciferase protein Renilla sp.

Protein Variants

Protein Variants Comment Organism
A55T/C124A/S130A/K136R/A143M/M185V/M253L/S287L mutant enzyme exhibits a greater than 4-fold enhancement in activity, a 200fold increased resistance to serum inactivation, and a small but measurable 5 nm red shift in the emission spectrum. The enhancement in light output arises from a combination of increases in quantum yield and improvedkinetics Renilla sp.
M185V/Q235A compared with the native enzyme the mutant has twice the rate of inactivation, as measured in murine serum, while incorporating a close to 5fold improvement in light output Renilla sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
 coelenterazine mutant enzyme A55T/C124A/S130A/K136R/A143M/M185V/M253L/S287L Renilla sp.
0.0029
-
 coelenterazine wild-type enzyme Renilla sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information recombinant enzyme is produced from a periplasmically or cytoplasmically expressed expression plasmid in Escherichia coli Renilla sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33800
-
 1 * 33800, mutant enzymeA55T/C124A/S130A/K136R/A143M/M185V/M253L/S287L, molar mass moment calculations based on the multi-angle scattering Renilla sp.

Organism

Organism UniProt Comment Textmining
Renilla sp.
-
 recombinant
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coelenterazine + O2
-
 Renilla sp. ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 33800, mutant enzymeA55T/C124A/S130A/K136R/A143M/M185V/M253L/S287L, molar mass moment calculations based on the multi-angle scattering Renilla sp.

Synonyms

Synonyms Comment Organism
RLuc
-
 Renilla sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.9
-
 coelenterazine wild-type enzyme Renilla sp.
4.9
-
 coelenterazine mutant enzyme A55T/C124A/S130A/K136R/A143M/M185V/M253L/S287L Renilla sp.