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Literature summary for 1.13.12.4 extracted from

  • Sun, W.; Williams, C.H., Jr.; Massey, V.
    Site-directed mutagenesis of glycine 99 to alanine in L-lactate monooxygenase from Mycobacterium smegmatis (1996), J. Biol. Chem., 271, 17226-17233.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Mycolicibacterium smegmatis

Protein Variants

Protein Variants Comment Organism
G99A (S)-lactate binds tighter to the G99A-mutant than to wild-type Mycolicibacterium smegmatis

Inhibitors

Inhibitors Comment Organism Structure
(R)-lactate competitive inhibitor Mycolicibacterium smegmatis
2-Hydroxy-3-butynoate suicide substrate Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
(S)-lactate calculated constant, G99A mutant Mycolicibacterium smegmatis
0.02
-
(S)-lactate observed constant, G99A mutant Mycolicibacterium smegmatis
0.055
-
O2 calculated constant, wild-type Mycolicibacterium smegmatis
0.071
-
O2 observed constant, wild-type Mycolicibacterium smegmatis
0.83
-
O2 observed constant, G99A mutant Mycolicibacterium smegmatis
0.92
-
O2 calculated constant, G99A mutant Mycolicibacterium smegmatis
22
-
(S)-lactate wild-type Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
8 * 43000, amino acid analysis Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + O2 Mycolicibacterium smegmatis
-
acetate + CO2 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + O2
-
Mycolicibacterium smegmatis acetate + CO2 + H2O
-
?
(S)-lactate + O2 G99A-mutant, no decarboxylation Mycolicibacterium smegmatis pyruvate + H2O2
-
?

Subunits

Subunits Comment Organism
octamer 8 * 43000, amino acid analysis Mycolicibacterium smegmatis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0148
-
(S)-lactate
-
Mycolicibacterium smegmatis

Cofactor

Cofactor Comment Organism Structure
FMN one FMN per subunit Mycolicibacterium smegmatis