Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Mycolicibacterium smegmatis |
Protein Variants | Comment | Organism |
---|---|---|
G99A | (S)-lactate binds tighter to the G99A-mutant than to wild-type | Mycolicibacterium smegmatis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-lactate | competitive inhibitor | Mycolicibacterium smegmatis | |
2-Hydroxy-3-butynoate | suicide substrate | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
(S)-lactate | calculated constant, G99A mutant | Mycolicibacterium smegmatis | |
0.02 | - |
(S)-lactate | observed constant, G99A mutant | Mycolicibacterium smegmatis | |
0.055 | - |
O2 | calculated constant, wild-type | Mycolicibacterium smegmatis | |
0.071 | - |
O2 | observed constant, wild-type | Mycolicibacterium smegmatis | |
0.83 | - |
O2 | observed constant, G99A mutant | Mycolicibacterium smegmatis | |
0.92 | - |
O2 | calculated constant, G99A mutant | Mycolicibacterium smegmatis | |
22 | - |
(S)-lactate | wild-type | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
8 * 43000, amino acid analysis | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + O2 | Mycolicibacterium smegmatis | - |
acetate + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + O2 | - |
Mycolicibacterium smegmatis | acetate + CO2 + H2O | - |
? | |
(S)-lactate + O2 | G99A-mutant, no decarboxylation | Mycolicibacterium smegmatis | pyruvate + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 43000, amino acid analysis | Mycolicibacterium smegmatis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0148 | - |
(S)-lactate | - |
Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | one FMN per subunit | Mycolicibacterium smegmatis |